The toxicity of caffein : an experimental study on different species of animals / By William Salant and J. B. Rieger.
- Salant, William, 1870-1943.
- Date:
- 1912
Licence: Public Domain Mark
Credit: The toxicity of caffein : an experimental study on different species of animals / By William Salant and J. B. Rieger. Source: Wellcome Collection.
Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.
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![Series A. In this series large dosea were administered, apjiniximatinK ()..S Kram per kilo. RdbhitJt. Grnij female. Weight, 1,659 grams. January 20: 2.20 p. m., 25 cc aqueous solution 2 per cent caffein (300 ni^ |)er kilo) were injected into the peritoneal cavity; 3.45 p. m., when doora of cage were opened rabbit had spasm of short duration. January 21: Rabbit found dead. Rabbit 7J. Gray and white. Weight, 1,402 grams. January 21: 11.17 a. m., 20.2 vv (acjueoiis solution) of 2 per cent caffein (300 mg per kilo) injected into i)eritoneal cavity from burette; 11.25 a. m., paralysis; 11.30 a. ni., rabbit had convulsion when picked up from the floor, followed by several epasms later; 11.35 a. m., typical tetanus; 12.30 noon, found dead. Rabbit 61. Black female. Weight, 2,14-3 grains. January 19: 40 cc 2 per cent caffein, aqueous solution (300 mg per kilo), injected into peritoneal cavity Ironi burette; tetanus when about 30 rr were injected; when removed from holder, repeated and vi«jlent convulsions, terminating in death. Series B. The object of these experiments was to determine the minimum lethal dose; 0.2 to 0.15 gram of caffein per kilo was injected into the rabbits of this series. Rabbit 69. White female. Weight, 1,714 grams. January 20: 10.15 a. m., (i cc 2 per cent caffein, aqueous solution, injected into peritoneal cavity. No symptoms, under observation for 45 minutes, rabbit defecated rather copiously; feces were soft; 11 a. m., 6 cc 2 per cent caffein, aqueous solution, injec-ted into peritoneal cavity, no symptoms, under observation for 40 minutes; 11.40, 6 cc 2 per cent caffein injected into peritoneal cavity; 11.45, rabbit is restless, reflexes increased. Rabbit 70. Gray and white female. Weight, 1,487 grams. January 20: 1.30 p. m., 15 cc 2 per cent aqueous solution of caffein (0.2 gram per kilo) injected into the peritoneal cavity; 2.20 p. m., no symptoms. January 30: About 2 p. m. rabbit died. February 1: Autopsy: Cirrhosis of the liver; enteritis of small intestines; stomach and kidneys normal. Rabbit 93. Maltese, male. Weight, 1,197 grams. March 2: 11.30 a. m., 12 cc of 2 per cent caffein (200 mg per kilo) injected into peritoneal cavity; 11.35 a. m., while being released from holder, tetanus occurred, repeated attacks later, clonic convulsions with tonic rigidity of posterior extremities during the attacks as well as during intervals, anterior extremities were relaxed during the intervals between the attacks, opisthotonos of cervical region but kyphosis in lumbar region were observed, no salivation nor dilatation of the pupils; 2 p. m., rabbit died. Rabbit 92. Yellow female. Weight, 1,388 grams. February 25, 4.15 p. m., 14 cc 2 per cent caffein (0.2 gram per kilo) injected into peritoneal cavity; 4.20 p. m., restlessness and increased reflexes, rabbit found stretched out in cage, but raised himself on his legs again; 4.45, general tremor when touched. February 26: 9 a. m., rabbit found dead. Autopsy: Hemorrhage into abdominal muscles at site of injection; hemorrhage also in walls of stomach opposite similar spot in abdominal wall. Rabbit,309. Belgian hare, female. Weight, 1,.500 grams. Diet, oats. March 2: 2.05 p. m., 2per cent caffein solution (0.2 gram per kilo) injected into peritoneal cavity; 2.25 p. m., found dead, no urine found in bladder. Rabbit 307. Belgian hare, female. Weight, 1,320 grams. Diet, oats. March 2: 12 noon, urine obtained from bladder, clear amber colored, no albumin, no reduction; 12.06 p. m., 10 cc of 2 per cent caffein (0.151 gram per kilo) injected into peritoneal cavity; 1.30 p. m., rabbit ])laced on floor, runs around but anterior and posterior extremities soon extended, in tonic condition; 2.25 p. m., reflexes increased, paralysis of extremities, dyspnoea; 4.50 p. m., about 100 cc urine collected, no albumin, reduction of Fchling's solution moderate. March 3: 9.30 a. m., posterior extremities extended and rigid, anterior extremities paralyzed, respiration less frequent and deeper than normal. Rabbit died at 11.50 a. m.; urine collected since 4.50 p. m. previous day gave very heavy reduction of Fehling's solution. Autopsy: Animal in good condition; in the left axillary region](https://iiif.wellcomecollection.org/image/b2120858x_0033.jp2/full/800%2C/0/default.jpg)
