A report on the microscopic objects found in cholera evacuations / by Timothy Richards Lewis.
- Timothy Richards Lewis
- Date:
- 1870
Licence: Public Domain Mark
Credit: A report on the microscopic objects found in cholera evacuations / by Timothy Richards Lewis. Source: Wellcome Collection.
Provider: This material has been provided by Royal College of Physicians, London. The original may be consulted at Royal College of Physicians, London.
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![selected for special f)1)S(n'vation, tlie one i'f;presoiitod at Eig. ix, 1; otluM- l)oiiig- added, the coiiloiits cleared ii]) a little, but Tiotliiug- I'Lirther; this was ibllowed by strong li(juor potassa^, AvMcli caused it to become dotted and streaky, the yellow tinge, hoAvever, remaining' ( 2 ) ; gi-a- Action of re-agents re- ^^^^^^ changing to thc app(^arance depicted at 3, the centre becommg more transparent than the circumference, wliich still lurtlic)' extended, as at 4. The transparency of tlie central portion diminished in the course of a few minutes (5), in which con- dition the object was left under the microscope until the next morning, when it was found to have retained its form, but liad acquu-ed a dark colour. Another cyst was selected witli a dark-yellow granular centre, and hyaline capsule (Kg. x, 1). Ether was added; scarcely any chauge; but no material change ob- jj^ercly clearing up the centre a little. ^^^^ ' It was then rolled over, and the granular contents spread throughout the entire cell (2). Firm pressure was applied, the eye being kept steadily at the microscope, when suddenly numerous minute molecules es- caped (3 ), and the capsule became Besuit of pressure. ^^^^-^^ emptied of its contcuts (4). Liquor potassEe was added to a portion of the evacuation and allowed to stand all night. The cysts on examination next morning appeared unaffected. To another test tube sidphmic acid was added. The cysts after remaining several hours in the acid were not much altered, but presented a globular outline with a hyaline capsLile smTounding a greenish-yellow molecular mass (Eig. xi, 1, 2). On rolling them over Action of sulphuric acid, bccamc oval, but soou regained iodine, and alcohol. the cii^cidar form. On the addition of a strong solution of iodine, the contents became dark-brown, and on subsequently adding absolute alcohol, fat-hke globules made theii' appearance, which, by mampulation, could be made to move mthin the cell; the capsvde bemg unaffected CFis xi 3) Alcohol being added to another cyst witliout the iodine, the contents assimied a lumpy appearance with a clear space in the centre (Eig.xi, 4). . Several very small embryos of round-worms having been observed in the evacuation in active motion (Eig. vm 8j, diligent search was made as to tlieu Embryos of worms asso- orioin, wliicli rcsultcd in the explana- ciatedwith the cysts. ^.^^ ^j^^ Cysts alsO.](https://iiif.wellcomecollection.org/image/b24749515_0034.jp2/full/800%2C/0/default.jpg)
