A report on the microscopic objects found in cholera evacuations / by Timothy Richards Lewis.
- Timothy Richards Lewis
- Date:
- 1870
Licence: Public Domain Mark
Credit: A report on the microscopic objects found in cholera evacuations / by Timothy Richards Lewis. Source: Wellcome Collection.
Provider: This material has been provided by Royal College of Physicians, London. The original may be consulted at Royal College of Physicians, London.
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![CULTIVATION OF CllOLliKAlC IJISCMAHGES To expeniiUMits condiicled in this mauiicir, there is the serious objection that each time the prcj)aratioii is examined, no matter how carefully, the possibility exists of foreio-n matter getting into the pre]iaration. With the intention'of T w . . obviating this source of fallacv as Isolating apparatus des- „ V, mi ^j. ia;j.ia.v.j an oribed. mucli as possiblc, an aspn-ator was em- ployed to sujjply the preparation with purified aii% at least as pure as passing it through concentrat- ed sulphuric acid will allow. By referring to the accompany- ing sketch, it mil be readily seen how this was effected (Eig. xviii). A small funnel (1) with a pledget of clean cotton wool inserted into its neck was attached to a piece of bent glass-tubing ; tliis tubing passed through a perforation in the cork of a flask (2) containing concentrated sulphm-ic acid; from the neck of this flask another piece of glass-tubing emerged which connected it with a perforated bell-glass, stand- ing in a shallow dish containing Condy's fluid; (3) another piece of tubing connected this with the aspirator (4) filled with water. All the connections were carefully luted, so that the only air which could have got at the preparation on the stand within the bell-glass (of course ?nmtis the air which previously existed therein) must have passed through the sulphm-ic acid. Illustration III:— A perfectly fresh choleraic evacuation having been obtained two hours before death (in a rapidly fatal case lasting only seven hours), three watch-glasses were placed in the isolating apparatus with the following ingredients :— No. I.—A slice of the interior of a plantain weighing quarter of an ounce was scooped out, Substances placed in the g-^ ^ q£ sediment fi'om the isolating apparatus J- evacuation was placed m the uttle cavity thus made. No. II.—A feAV drops of the evacuation-sediment only. No. III.—A slice of the same plantain as in No. I. The apparatus had been made as clean as possible pre- vious to this, rinsed out with spu-it hnmediately before deposit- ing these glasses on the stand beneath the bell-glass, and the gi-eatest care taken to avoid foreign matter getting at the pre- parations before placing them there. The an- Avithm was renewed morning and evening; the weather was warm the whole time, the average day fcemperatm-e of the room bemg about 90° Fahr.](https://iiif.wellcomecollection.org/image/b24749515_0042.jp2/full/800%2C/0/default.jpg)
