The action of alcohols and aldehydes on proteid substances / by T. Lauder Brunton and Sidney Martin.

  • Brunton, Thomas Lauder, Sir, 1844-1916.
Date:
1891
Catalogue details

Licence: Public Domain Mark

Credit: The action of alcohols and aldehydes on proteid substances / by T. Lauder Brunton and Sidney Martin. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    THE ACTION OF ALCOHOLS AND ALDEHYDES ON PROTEID SUBSTANCES. By T. LAUDER BRUNTON, M.D., E.R.S. and SIDNEY MARTIN, M.D. In our experiments, we have tested the effect of the series of liquid alcohols on proteid substances, in order to determine whether those higher in the scale differed from those lower. It was a priori possible that the results obtained might have some bearing on the physiological action of the alcohols and aldehydes. The solutions of proteid used: were (1) egg-albumin, con- taining no globulin, (2) serum-albumin of the sheep, containing a trace of serum-globulin, and (3) a mixture of proto- and deutero- albumose, prepared from Witte’s “Pepton.” The method adopted was to drop the solution of proteid from a pipette into six times its bulk of the reagent: thus if 10 c.c. of solution were used, it was dropped into 60 c.c. of alcohol or aldehyde; if 5 c.c., it was dropped into 30 c.c. The action was tested in the following manner :— a. It was first noticed whether the reagent precipitated the proteid or not. /3. Whether the precipitate, if it occurred, was rendered insoluble after remaining some time under the alcohol or aldehyde. The solu- bility after treatment was tested by filtering off the precipitate, allowing the reagent to evaporate from the proteid, and testing how far this was soluble in distilled water. 7- Whether any change of colour occurred in the precipitate. Action on Egg-albumin. Of Alcohols. Ethyl 1 Precipitate egg-albumin and coagulate almost completely Propyl J in 4 clays. 3. Methyl Same as above, but coagulation not so complete. 4. Isobutyl ] r> . . , t ( , I Precipitate and coagulate, but to less extent than Te™ Butyl J |,recedin*' ‘fter 4 ^ 1. 1. 2. 5. 6.