Studies on the proteids of rye and barley and on the chemical nature of diastase.

  • Thomas Burr Osborne
Date:
[1893]
Catalogue details

Licence: Public Domain Mark

Credit: Studies on the proteids of rye and barley and on the chemical nature of diastase. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    in coagulum formed until the solution was boiled and then only a small part of the dissolved substance sepai-ated. Three kilos of barley meal were extracted with 10-per cent, salt solution, the filtered extract saturated with ammonium sulphate and the resulting precipitate filtered out, dissolved in 10-]3er cent, brine and the insoluble matter removed by filtration after adding a very small quantity of two-tenths-per cent, potash water in order to neutralize the slight acid reaction of the extract. The solution was then filtered clear and dialyzed for four days. The proteid separated in the form of small spheroids which were fil- tered out, washed with water, alcohol and ether, and after drying over sulphuric acid found to weigh 4.02 grams. This preparation was dissolved in 10-per cent, salt solution and again submitted to dialysis. After the proteid had precipitated it was filtered out, washed with water, alcohol and’ ether, and the final preparation, when dried at 110°, had the following composition: Barley Globulin, Edestin, Preparation 7. Carbon Hydrogen Nitrogen . Sulphur 1 Oxygen \ 51.43 6.11 18.14 •23.72 Ash 100.00 0.48 Again six kilos of barley flour were extracted with 10-per cent, salt solution, the filtered extract saturated with ammonium sul- phate, the resulting precipitate dissolved in salt solution and dialyzed. The precipitated globulin was again dissolved in 10-per cent, salt solution and precipitated a second time by dialy- sis. 1.9 grams of preparation 8 were obtained, having the fol- lowing composition; Barley Globulin, Edestin, Preparation 8. 50,32 Hydrogen 6 7g Nitrogen Sulphur I Oxygen [ - 24.26 Ash 100.00 0.37