Transfected glioblastoma cells: reduced endocytosis

Human glioblastoma cells that have been transfected with a mutated P10 gene. This produces a catalytically inactive P10 protein. These particular glioblastoma cells have no natural P10 so when the cells are transfected with a functional P10 gene, mobility and endocytosis are reduced. Transfection with an inacative P10 gene acts as a control for experiments where another batch of cells are transfected with a functional P10 gene. P10 is a tumour suppressor gene that is mutated in many cancers. This mutation leads to increased cellular mobility and may play a role in metastasis of the cancer. Nuclei are stained blue with DAPI and the transfected cells are full of Green Fluorescent Protein. The red spots are 20 nanometre fluorescent spheres used to observe the process of endocytosis. Some of these spheres have been taken up by the cells.