Micro-organisms and disease : an introduction into the study of specific micro-organisms / by E. Klein.

  • Klein, E. (Edward), 1844-1925.
Date:
1885
Catalogue details

Licence: Public Domain Mark

Credit: Micro-organisms and disease : an introduction into the study of specific micro-organisms / by E. Klein. Source: Wellcome Collection.

Provider: This material has been provided by Royal College of Physicians, London. The original may be consulted at Royal College of Physicians, London.

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    below), then washed for a few seconds with a 10 per cent, solution of nitric acid, then for a few minutes with distilled water. After this the preparation is stained with methyl- bine in the ordinary way. Or, after Koch's method, the specimen is first stained in alkaline methyl-blue (mixed with a 10 per cent, solution of caustic potash) for twenty-four hours, or for half an hoi;r to one hour at a temperature of 40° C, and then stained in a concentrated solution of vesuvin. Wash it next with water, then Avith alcohol, dry, and mount in Canada-balsam solution. In leprosy, the s]jecimen on the glass is stained with magenta, then washed in distilled water, then stained with methyl-blue, washed, and mounted. With such organ- isms—as e.g. the micrococcus in the sputum of acute croupous pneiimonia, and the micrococcus in gonorrhoeal discharge— the staining is best carried out with a mixture of methyl-blue and vesuvin. Weigert's double stain is very excellent for many purposes ; it is prepared thus :— y Saturated watery solution of aniline, 100 ccm. [This is made thus :—Aniline oil, 1 part, dist. water, 3 parts. Shake every half hour for four hours, and decant the water as the oil settles to the bbttom.] Saturated alcoholic solution of fuchsin, 11 ccm. Mix. The sections are well stained in this mixture, then washed in distilled water ; after this they are immersed for a few seconds in alcohol, and then transferred for one, two, or three hours to the following solution [Watson Cheyne, Practitioner, April 1883, p. 258] :— Distilled water, 100 cbm. Saturated alcoholic .solution of methyl-blue, 20 ccm. Formic acid, 10 minims. After this, wash in alcohol, pass through clove-oil, and mount in Canada-balsam solution. In examining fresh or hardened tissues for micro-organisms it is necessary to make thin sections, which can be easily done with the aid of any of the microtomes in common use, amongst which Williams's microtome, and especially Dr. Koy's ether-spray freezing microtome, are no doubt the best and easiest to manipulate. As regards hardened material, it is necessary to remember that tlie hardening must be earned
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