Creatin and creatinin / by Edward Mellanby.

  • Edward Mellanby
Date:
[1908?]
Catalogue details

Licence: In copyright

Credit: Creatin and creatinin / by Edward Mellanby. Source: Wellcome Collection.

    18/42 page 464
    The above were allowed to stand with toluol, in water bath at 87° C. for 48 hours. Proteids as before were got rid of by 60 per cent, alcohol and in evaporation the temperature was kept below 50° C. The following quantities of creatin and creatinin were estimated : Creatin (estimated as creatinin) Creatinin A 82 mgrs. — B 81 — C — 31*5 mgrs. D — 31-25 E — 31-25 F _ no creatinin From these results it is apparent that the creatin and creatinin are unaffected by extracts of rabbit’s liver and muscle. It seemed possible that the absence of results confirmatory of Gottlieb and Stangassinger might be due to the fact that most of their experiments were done on cats and dogs. It has been shown by various experimenters how very variable is the susceptibility of uric acid to the tissues of different animals. For instance Wiener (35) has shown that extracts of the livers of dogs and pigs can destroy uric acid while the livers of herbivorous animals increase the quantity rather than destroy it. In order to avoid this possible source of difference the following experiment was done. Exp. 8. Cat. The liver was cut out immediately after death and ground up with ice- cold Einger solution. After standing for half an hour, the liquid extract was added to solutions of creatin as follows : Pure creatin solution Ringer Extract of liver A 10 c.cs. 50 0 B 10 25 25 C 10 25 25 The above solutions were allowed to stand for one week at 37° C. with toluol. The following amounts of creatinin were estimated after conversion of the creatin : A. 35-5 mgrs. B. 35 mgrs. C. 34 mgrs. No creatinin could be found before conversion of the creatin, i.e. in the extract after evaporation below 50° C. Creatin is therefore uninfluenced by an extract of cat’s liver. Exp. 4. Hedgehog. The liver was ground up with Einger’s solution and added to solutions of creatinin as below : Creatinin Liver extract A 5 c.cs. 50 C.CS. B 5 50 C 5 50 C was first boiled. A little toluol was added to each and they were then allowed to stand at 37° C. for three days. At the end of this time the proteids were precipitated by
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