Creatin and creatinin / by Edward Mellanby.

  • Edward Mellanby
Date:
[1908?]
Catalogue details

Licence: In copyright

Credit: Creatin and creatinin / by Edward Mellanby. Source: Wellcome Collection.

    22/42 page 468
    When dealing with the question of the methylation of glycocyamin it was seen that whereas Jaffd got an increase of creatin in the muscle of full grown rabbits after feeding with this substance, I could obtain no increase when young chickens with their muscles unsaturated by creatin were fed with glycocyamin. The method of experiment described has been proceeded with and there will now be set out some results obtained by feeding young chickens on creatin. The chief reason that chickens were chosen in these experiments was because they are easily fed when young. Exp. 1. 191 chickens born July 13th to 14th, 13 were fed on one gram creatin from July 21st to July 25th, in addition to their ordinary food, which was quite free from creatin. Six control chickens received only the creatin free food. All the chickens were killed July 26th, and the muscle dissected off. Great trouble was taken to free the muscle of all visible fat. The 13 creatin fed chicks were divided into two lots X and Y, and their creatin estimated by the previously described method. Muscle Results ground up 6 control chickens 28’1 grs. 6 creatin fed chicks X 33*5 7 creatin fed chicks Y 36*2 Total weight Percentage creatinin (from of chicks creatin) to muscle 283*5 grs. 330*2 372 •30 p.c. *295 •285 Exp. 2 15 chickens born August 12th and 13th. A. 5 fed on creatin free diet. B. 5 fed on creatinin and ordinary diet. C. 5 fed on meat extract, and ordinary diet. The creatinin and meat extract2 were given to the chicks from August 20th to 25th. About 1^ grs. creatinin in solution were given to B chicks. The chickens were killed August 26th, i.e. a fortnight old. The meat extract contained mostly creatin but also contained a little creatinin. The creatin was not estimated but the extract represented about 400 grs. of rabbit’s muscle. Weight of Average Percentage creatinin Results chicks weight obtained from muscle A. Control 257 grs. 51*4 grs. B. Creatinin fed 282*5 56-5 C. Meat extract (i.e. mostly creatin) 264 53 •287 p.c. •297 •293 It is seen that in the above experiments feeding with creatin or creatinin produce no apparent increase in the percentage creatin content of muscle. If creatin feeding in young animals has no such effect on chickens at this age, it seems more improbable that glycocyamin given in the food will be methylated and stored in muscle as creatin. 1 Most of the chickens used in this research were supplied by Mr W. Bateson, to whom my thanks are due. 2 The creatin, creatinin, meat extract and glycocyamin, according to the experiment, were always introduced directly iptg the ghickeij’s stomach by means of thin indiarubber tubing,
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