Creatin and creatinin / by Edward Mellanby.

Edward Mellanby

Date:: [1908?]

Credit: Creatin and creatinin / by Edward Mellanby. Source: Wellcome Collection.

23/42 page 469

$It was thought probable that the above negative results might be due to the fact that the muscle of any animal can hold only a definite amount of creatin, which cannot be increased and that in the time of living, i.e. a fortnight, the chickens’ muscle had nearly reached this limiting point. The muscle of a hen was examined and found to contain '31 per cent, creatinin (from creatin). In the following experiments the chickens were only allowed to live a few days. Exp. 3. 12 chickens hatched out September 6th and 7th. Killed September 12th, i.e. 5 days old. On September 10th and 11th, one gram of pure creatin was given them by mouth. They were divided into two lots and the creatin estimated in the muscle of each. Results Weight of chicks Average weight Muscle ground up Percentage creatinin from creatin in muscle A. 6 chicks 243 grs. B. 6 chicks 262*5 6 control chicks 256*3 40*5 grs. 43*7 42*7 20*48 grs. 21*18 2207 *275 p.c. •264 *238 1 gram creatin. creatin free diet. These latter control chicks were born September 17th and killed September 22nd, i.e. 5 days. The result of this latter experiment would certainly point to an increase in the creatin of muscle as a result of giving this substance by the mouth. Exp. 4. 16 chickens born Sept. 17th. Killed Sept. 21st, i.e. 4 days old. On Sept. 19th and 20th, 6 were given \ gr. pure creatin in 50 c.cs. water. A. 10 ,, 1*2 grs. creatinin ,, 75 ,, ,, B and C. These substances were given by the mouth, a little at a time, about five times a day. The 10 creatinin fed chicks were divided into two lots, B and C. Immediately on killing the chickens, the creatin was estimated in the muscles, the latter being carefully deprived of visible fat. Results Total weight Age Average weight Muscle ground up Percentage creatinin estimated A. 6 chicks 262 grs. 4 days 43*7 grs. 20*6 grs. *257 p.c. (creatin fed). B. 5 chicks 222 4 44*4 16*72 •246 (creatinin fed) C. 5 chicks 231 4 46*2 18*1 *257 In a control set of 6 chicks, which were killed Sept. 22nd, and were thus one day older than above chickens, the estimated creatinin was *238 per cent. Exp. 5. 10 chickens born Friday, Sept. 27th. Killed Sunday, Sept. 29th, i.e. 2 days old. On Sept. 28th, 5 chickens A were given 300 mgrs. of creatinin in 20 c.cs. water, in addition to other food. The other 5 chicks B were fed on creatin free diet. Results Weight of muscle ground up Percentage creatinin estimated Age A. 5 creatinin-fed chicks 18*78 grs. *205 p.c. 2 days B. 5 control 17*15 *187 2$

Creatin and creatinin / by Edward Mellanby.

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