Clinical diagnosis : the bacteriological, chemical, and microscopical evidence of disease / by Rudolf v. Jaksch ; translated from the second German edition by James Cagney ; with an appendix by Wm. Stirling.

  • Cagney James.
Date:
1890
Catalogue details

Licence: Public Domain Mark

Credit: Clinical diagnosis : the bacteriological, chemical, and microscopical evidence of disease / by Rudolf v. Jaksch ; translated from the second German edition by James Cagney ; with an appendix by Wm. Stirling. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

    76/432 (page 48)
    Bernard is the best. This consists in addin to it its own weight of crystalline sodic sulphate, boiling, and filtering. [See Appendix VIII.] The filtrate is free from proteids, and may be tested for sugar thus : 1. Moore's test Avill serve where sugar exists in any quantity. (See chapter on the Urine.) 2. Trommer's test. (See chapter on the Urine.) 3. The phenyl-li.ydrazin hydrochloride test is the best for detecting o slight traces of sugar in the blood. It is conducted as follows {v. Jalisch): — Add together two parts of phenyl-hydrazin hydrochloride and four parts of acetate of soda; add water and heat. Take 5 cc. of the proteid-free filtrate (which is practically a saturated saline solution), obtained by Claude Bernard’s process, and while still warm add it to 5 cc. of the solution prepared as above. Place the mixture in a test- tube half filled with water, heat it for half-an-hour on a water-bath, and allow it to stand. Or a little of the phenyl-hydrazin salt and acetate of soda may be added in the dry state to the warm proteid- free filtrate, and the process conducted as described above. After it has cooled, when examined under the microscope, it is seen to contain separately and in clusters the characteristic yellow crystals of phenyl- glucosazon scattered amongst colourless crystals of sulphate of soda. (See chapter on the Urine.) To determine the percentage of sugar in the blood, Felding's fluid may be employed (the blood having been previously freed from proteids) in the manner afterwards to be recommended for testing for sugar in the urine, and the polarimetric test * may be applied. It seldom hap- pens, however, that the filtrate contains sufiicient sugar to be appreci- able with the polarimeters at present in use. instrument is the most sensitive, and gives the best results in this connection. In diabetes large quantities of grape-sugar are found in the blood. Hoppe-Seyler describes a case in which it reached as high as o. 9 per- cent. The researches of Freund^^'^ would make it appear that a deoxi- dising .substance—presumably sugar—exists in considerable quantity in the blood in cases of carcinoma. [This statement, however, needs further confirmation.] The blood of tubercular patients is said by the same observers to contain cellulose.^®^ For the detection of sugar and the carbohydrates generally in the blood, the processes of Baumann and Udranslnj'^^'^ may be employed with advantage. These are based upon the fact that the carbohydrates are precipitated from their w'atery solutions by the addition of benzoyl chloride and caustic potash, forming insoluble compounds. This com- bination of the carbohydrates with benzoyl chloride may easily be * See chapter 011 the Urine.