Determination of environmental safety of Agrevo Canada Inc.'s glufosinate ammonium-tolerant canola.

  • Agriculture and Agri-Food Canada
Date:
1995
Catalogue details

Licence: In copyright

Credit: Determination of environmental safety of Agrevo Canada Inc.'s glufosinate ammonium-tolerant canola. Source: Wellcome Collection.

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    HCN92 was observed, including brood development. Both enzymes are rapidly inactivated in mammalian stomach and intestinal fluids by enzymatic degradation and pH-mediated proteolysis. Neither of the two novel proteins contained potential glycosylation sites nor did they possess proteolytic or heat stability, indicating that neither protein is a likely allergen. A search of the GENEBANK DNA sequence database revealed no significant homology with the toxins or allergens entered in that database. Based on the above, AAFC has determined that the unconfined release of HCN92 will not result in altered impacts on interacting organisms, including humans, compared with currently commercialized counterparts. | 5. Potential Impact on Biodiversity HCN92 has no novel phenotypic characteristics which would extend its use beyond the current geographic range of canola production in Canada. Since outcross species are only found in disturbed habitats, transfer of novel traits would not impact unmanaged environments. Studies have shown to AAFC that HCN972 is not invasive of natural habitats, and that it is no more competitive than its counterparts, both in natural and managed ecosystems. AAFC has therefore concluded that the potential impact on biodiversity of HCN92 is equivalent to that of currently commercialized canola lines. V. Assessment Criteria for Use as Livestock Feed 1. Anti-Nutritional Factors Ninety-five percent confidence intervals were determined for glucosinolate and erucic acid content of the meal and oil produced from HCN92, grown under a variety of conditions. These confidence intervals demonstrated that the PNT contained levels of these anti-nutritional factors below the prescribed standards for both the meal and oil fractions, i.e., <30 micromoles glucosinolates per gram of dry meal and <2% erucic acid in the oil. 2. Nutritional-Composition of PNT No statistical differences in nutritional composition, i.e., crude protein, crude fat, crude fibre, ash and gross energy content, were noted between the whole seed, processed meal or oil of HCN92 and current commercial canola cultivars. These results collectively demonstrate that the introduction of this construct into B. napus, resulting in HCN92, did not likely result in any secondary effects impacting on the composition or nutritional quality of the cultivar. Accordingly, HCN92 was judged to be substantially equivalent to traditional canola varieties in terms of nutritional composition.