Microscopical morphology of the animal body in health and disease / by C. Heitzmann. With 380 original engravings.
- Carl Heitzmann
- Date:
- 1883
Licence: Public Domain Mark
Credit: Microscopical morphology of the animal body in health and disease / by C. Heitzmann. With 380 original engravings. Source: Wellcome Collection.
Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.
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![the rim of varnish, and the siH'cinicn l)e('<)nies si)()iled. As an inchtsinir varnish, asplialt dissolved in tnrpentine is f^enerally used, thonjj^h any ntlicr vai-nish answers the pnrpose if ])ut on in suffieient (piawtity. The ni()untin<i: and varnisliinj; «»f •glycer- ine specimens is easier with sc^nare than with circnlar covering- classes. Sf<iiiiinf/. The amnioniacal carininc solnti<)n ((lerlach) is the most satisfactory for stainini^ specimens obtained after hardening in chi-omic acid solution. To the best cochineal powder we add distilled water and a few di'ops of aqua ammoniap fortis, until the cochineal is c(mipletely dissolved. The amount of the car- mine solution to be poured into the saucer holding the sections depends on the concentration of the solution. The best way is to take but little carmine, and let it act on the specimen for twenty-four hours. The various compounds of carmine in use may be dispensed with, as all carmine staining is very unreliable, and, except for the handsome appearance it gives to the speci- men, of no material valiu\ HamiatoxyL)n (logwood) and eosine are re-agents used for alcohol specimens exclusively, but not suitable for chromic acid preparations. The action of the picric acid is kindred to that of chromic acid. AniUne colors as a rule are not fast, neither are solutions of picro-indigo. Osmic acid (M. Schultze) in a one per cent, solution stains fat black in both the fresh and the preserved condition of the speci- men ; it renders the c( )ntours of the tissue, especially nervous tissue, more distinct, but other-wise has a very limited value. Important re-agents are the nitrate of silver (Von Reckling- hausen) and the chloride of gold (Cohnheim); though specimens treated \nth either of these re-agents become indistinct after five or six years. Nitrate of silver is brought into contact, in a one per cent, or two per cent, solution (kept in black bottles), exclu- sively with fresh specimens, for only a few minutes, or used for injections into blood and lymph vessels. Distilled water is needed for washing off the re-agent. The solid nitrate of silver may be applied directly on dense tissues, such as cornea or cartilage, though the layers which come in direct contact with the stick are destroyed. Silver-stained specimens are suitable for glycer- ine mounting. Chloride of gold is invariably used in a one-half per cent, solution, and is fit for fi-esh and frozen specimens, as well as for those preserved in chromic acid; in the latter case, after careful](https://iiif.wellcomecollection.org/image/b21219163_0035.jp2/full/800%2C/0/default.jpg)
