Recent studies of the olive-tubercle organism / by Erwin F. Smith.

  • Erwin Frink Smith
Date:
1908
Catalogue details

Licence: In copyright

Credit: Recent studies of the olive-tubercle organism / by Erwin F. Smith. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Hah.: In tumorilms Olcae ciiropeae. Coloniae in gelatina tenues, albae, niarginibus inaeiiuales, nonliquefaeientes. Coloniae in ajjar- apar alliae, rotundae, nitentes, evolventes lente (in extremo die sep- timo 2-5 nun. latae). Culturae in tul)eribus Solani prime alliae, dein pallidae fulvae-albae sunt. Lac sterile alealinum fit et casein non- segregatur. Bacidi metbodo Gram noncolorantiu*- Nitmm non- redigitur. Acidum in mediis cum saccharo uvae celeriter fit. Si cul- turae novae in infusione carnis } horam in temperatura 50° C. tenen- . tur, moriuntur. Inter tcmperaturam 35° C. et temperaturam 1° C. crescit. Inoculatum in Oleas sanas, tumores jirojirios producit. A white, nonliijueh'ing scbizomycete, causing olive tubercle. This organism is found in the olive tubercle and in many culture media as a short rod with rounded ends, either single or in jiairs, growing end to end, or in small clumps,more rarelyinshort chains; the rodstaken from the interior of an unruptured tubercle measured 1.5 to 3 X 0.6 to O.S/i when stained by carhol-fuchsin and washed in water; the rods from 3'oung agar cultures staineil with carhol-fuchsin usually measure about 1.2 to 1.5 X 0.5/<; rods from 3 days’ old colonies on agar mea.sured 1.2 to 2 X 0.4 // when stained h}' a modified Gram, i. e., washed in anivl alcohol, and cleared in xvlol; more rareh' they' are 10/I or more in length; in bouillon ke]>t fi)r three days at 30° C. the thinh' clouded fluid examined in hanging drops contamed numerous actively motile rods five to twentv-five times as long as broad, some of the long rods ]>lainlv con.stricted in the middle, others not, the long ones flexuous;“ the organi.sm is motile b}* means of one to several polar flagella, often 2 to 4 (Pitlield’s flagella stain), the rods so stained being 2 to 5 X 0.6 to O.S //. Young motile agar cultures stain readily with carhol-fuchsin, but noth}'Gram; the organism is aerobic and verA'.sensitive to heat; the growth is white in various culture media (bouillon, ])c])tone water, milk, standard agar, sugar agars, gelatin (especiallA' at bottom of .slant), silicate jelh*, starch jelh', Cohn’s .solution, Fermi’s solution, U.schin.sky’s solution); on -M5 nutrient agar in poured ])lates made from bouillon and incubated at 20° C. or 25° C., the surface colonies are .small, circular, smooth, glistening (internal structure reticular), rather slow growmg, appearing at the end of the second and third ihn's and best ol)served after three to four daj's, becoming denser ami whiter with age;* the intruders usualh' come uji first in jilates made “ In a repetition maile in 1908 the s;une re.“!iilt was obtained; Tlie longest single rods were 3//; the chains varied from G to 40//; some of the chains were actively motile- 6 In -flo standard nutrient agar in Petri-dish poured plates made recently and ke])t at 22° to 23° C. the surface colonies in thin-sown plates were pure white, rather slow growing (1.5 to 3 nun. at the end of the third day), round, flat, surface smooth, glisten- ing, edge entire or nearly so, internal structure under the compound microsco])0 amorphous to finely granular; l)uried colonies rpiite small. -\t the end of seven dal's