Recent studies of the olive-tubercle organism / by Erwin F. Smith.

  • Erwin Frink Smith
Date:
1908
Catalogue details

Licence: In copyright

Credit: Recent studies of the olive-tubercle organism / by Erwin F. Smith. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    days; it l)lues litmus milk, the nuid, which is lavender or lilac on the start, hecomin;]: gradually a deep blue; it tloes not form acids in milk and does not coagidate milk hy a lah ferment ; it grows readily in Cohn’s solution and for a long time, jiroducing an abundance of crv.s- tals of ammonium magnesium ])hosphate, no difficulty being e.xperi- enced in obtaining enough for a chemical analysis from small flask cultures; in old gelatin streaks and in old gelatin stab cultures there H was a thin white surface layer, hut no trace of liciuefaction, growth along the line of the stabs was not well develojied; in gelatin streaks and gelatin colonies there is an irregular undulatory lohed or incised margin; organism non.sporiferous, nonlitiuefving, nongas forming; ' growth on cooked jiotato at the end of two days was smooth, white, wet-shining, distinct, hut ii(»t ver}' copious, cylinder slightly grayed and fluid at the bottom moderately cloudy, the growth at first on > ])otato being not unlike the ]mre white growth of Bacillus- irachei- pliilus, evi'ii in old cultures the growth is never a decideil yellow (see footnote); jiotato cultures eighteen days old gave a deep puqdish ; blue reaction when mashed in iodin water, the checks giving a bright | blue color, i. e., the organism acts hut slightly on jiotato starch; action on olive starch unknown; it does not reduce nitrates in pep- tonized beef bouillon; it will not grow in beef bouillon at 38.5° C., and is killed in + 15 standard beef bouillon by e.xpo.sure in the water hath, in test tubes, for ten minutes at 50° ; streak cultures on litmus agar's containing filtered river water and Witte’s jieptone, hut . free from beef juice, behave as follows; Plain litmus agar.—This becomes blue and remains .so (forty days), ■ growth moderate. | Litmus dextrose agar.—This reddens jmnnptly (twenty-four hours, \ at 27° ('.) and decidedly, and remains acid indefinitely (color bright reil—not purjile), growth slight. *. Litmus galactose agar.—This reddens ])romptly*and decidedly and ; remains acid (forty days); same reaction as with litmus dextrose agar, growth moderate. Litmus saccharose agar.—This remains neutral or nearly so for some days, finally, however, becoming jnirplish and then jnirple-red; growth stimulated, i. e., a much greater volume of bacterial slime than on the other agars and the litmus reduced in the bottom of the tube after some days. ■■ Litmus lactose agar.—This blues; growth not more than on the plain litmus agar; no acid was formed (thirty days). Litmus maltose agar.—This blues, growth moderate; no acid was formed (thirty da3”s), and there was no reduction. Litmus mannit agar.—This blues, growth moderate, after ten da.ys litmus purplish, never red.