The routine preparation of diphtheria toxin / by Percival Hartley and Olga Mary Hartley.

  • Percival Horton-Smith Hartley
Date:
[1922?]
Catalogue details

Licence: In copyright

Credit: The routine preparation of diphtheria toxin / by Percival Hartley and Olga Mary Hartley. Source: Wellcome Collection.

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    Table I. Showing results of diphtheria toxin production from November 1919 to May 1920. Number of Toxins. Volume of Toxins. Minimum Lethal Dose. Percentage of Total Volume of Toxin made. 3 Litres 33 c.c. o-ooi 3 17 446 0-002-0-0011 44 13 225 0-003-0-0021 22 7 183 0-004-0-0031 18 2 55 0-005 5 1 35 0-0055 3 1 35 0-0075 3 Totals 44 1012 By arranging the results in a slightly different way (Table II.), it is possible to compare the above figures with those obtained by Dean (1908 4) and Davis (1920 s). Table II. Comparison of the results obtained by Dean (1908) and Davis (1920) and the authors. M.L.D. c.c. Dean, Series 1, 1S99-1902. Dean, Series 2. 1904-1905. Davis, 1920. P. and O. M. Hartley. No. of Toxins. Per cent. No. of Toxins. Per cent. No. of Toxins. Per cent. No of Toxins. Per cent. 1/1000 0 • • • 0 \ 3 7-0 1/800 0 • • • 0 • • • 1 2-2 1/700 0 1 2-6 1 2-2 1/600 0 • • • 1 2-6 ( 43-0 6 14-0 1/500 2 2 8 20-5 9 20-0 1/400 14 15 9 23*1 10 23-0 1/300 17 18 7 18-0 35-5 10 23-0 1/200 17 18 7 18-0 11-3 3 7-0 1/100 21 22 1 2-6 10-2 1 2-3 >1/100 16 17 • • • \ • • • • • • • . • • • • “ other toxins ” 7* 8 5f 13-0 • • • ... • • • Not Totals . 94 ... 39 ... stated ... 44 ... * M.L.D. not accurately determined, but greater than 1/300 c.c. t M.L.D. not accurately determined, but greater than 1/200 c.c. Dean used a 2 per cent, solution of Witte’s peptone in beef infusion, made the broth (while still hot) neutral to litmus paper and then added 7 c.c. of normal caustic soda for every litre. Davis used a 2 per cent, solution of Parke Davis Bacteriologic Peptone in beef infusion, the reaction being adjusted to PH = 8,0 to 8-2. We used a method differing only in detail from that of Davis. The strain employed in the four series of results analysed in the above table was Park Williams No. 8 A 2
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