Investigations into the growth of the lymphosarcomata in dogs / by John W. Hunter, Geo. M. Laws and Leo Loeb.

  • Hunter, John W.
Date:
1909
Catalogue details

Licence: In copyright

Credit: Investigations into the growth of the lymphosarcomata in dogs / by John W. Hunter, Geo. M. Laws and Leo Loeb. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    sarcoma was removed from a dog, and three pieces were inoculated into each of four new dogs (1,2, 3, and 4), two sujiposedly immune dogs (5 and 6), and one dog in which a tumor was already growing (7). After four days, oue of these transplanted masses was removed from Dogs 1, 2, 3, 4, and 7. Abscesses, but no tumor masses were found in Dog 5. No tumor masses could be determined in Dog 0. Tumor from Dog No. 1: Microscojiically, this mass was mostly necrotic. In the peri])hery around the blood- vessels proliferating cells were seen, but these were not typical tumor cells. A number of polynuclear leukocytes were present in the centre and periphery. The proliferating cells may have been either tumor cells or fibroblasts. Tumor from Dog No. 2: IMicroscopically, this mass was mostly necrotic. In the periphery living tumor cells with mitoses were still preserved. These were arranged in nests. Among them we saw spindle-shaped cells, stellate and round cells, and polynuclear leukocytes. Quite outside of the above zone of tumor cells were to be seen vessels with proliferating cells around them, as in the mass from Dog No. 1. In some places we saw nests of tumor cells surrounded by fibrin, but no connective tLssue. In other places connective tissue surrounded the nests. There was, if anything, more active proliferation of the connective tissue at a distance from the tumor tissue than in its immediate neighborhood. There were some irregular mitoses among the tumor cells. Quite a number of polynuclear leuko- cytes could be made out among the tumor cells, between them and the connective tissue, and also in the connective tissue. From examination of these sections there was no reason to believe that the connective tissue proliferation gave rise to the tumor. d'umor from Dog No. 3: dliis nia.ss showed a necrotic centre surrounded by living tumor tissue, but separated from it by a slight zone of connective tissue growing into the necrotic tumor tissue. Near the living tumor tissue, here and there, large cells (phagocytes) which took up the