A manual of histology / edited and prepared by Thomas E. Satterthwaite, in association with Thomas Dwight [and others].

Date:
1881
Catalogue details

Licence: Public Domain Mark

Credit: A manual of histology / edited and prepared by Thomas E. Satterthwaite, in association with Thomas Dwight [and others]. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    2G of silver, and, half an hour afterward, immerses it in water acidulated with acetic acid; after a day or two it is found to have a glutinous appearance. The lamella; are then easily stripped off, and in the middle portions, the corneal corpuscles assume a purplish-brown color while their nuclei are uncol- ored. The outlines of the lymphatic channels are also sharply defined. Picro-hcematoxylin and eosine (trijple-staining).—Wendt has described a method of double-staining by picric acid and lnematoxylin. Only the very thinnest sections, however, give satisfactory results. A strong solution of limmatoxylin is first erupted. In this the sections are allowed to remain about twelve hours. After washing them in water, they are placed in a saturated solution of picric acid and carefully watched. They may be removed from time to time, examined with a low power, and, when properly stained, put in alcohol and mount- ed in Canada balsam with as little delay as possible. To ob- tain triple-staining, eosine may be conveniently combined with this picro-hsematoxylin method. To insure good results some amount of practice is necessary. Double, triple, and quadruple staining.—Dr. Gibbes re- commends for double-staining, immersion first in picro-carmine and then in logwood, or which is better, immersion first in a spirituous solution of rosine or aniline violet, and then in an aqueous solution of aniline blue or iodine green. In obtaining more than two colors there is considerable difficulty. To ac- complish it he uses first the chloride of gold or picro-carmine and then the spirituous and aqueous solutions of the ani- lines. Staining with Bismarlc brown.—Make a watery solution of gr. ij.— Ij., heat and filter; soak in the solution about three minutes ; set the color with acetic acid (glacial) 4 per cent, for half a minute. After dehydrating with alcohol mount in dammar varnish. Weigert prepares the Bismark brown as follows : he makes a concentrated aqueous solution by boil- ing in water, filtering from time to time. He also uses a weak alcoholic solution, and combiues with other colors. [To combine with eosine—put the sections in a strong aqueous solution of Bismark brown; remove after about two minutes, set in weak acetic acid (four per cent.), then place in a weak alcoholic or aqueous solution of cosine, and then again in the acetic acid solution.—T. E. S.]
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