The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.
- Arthur Bolles Lee
- Date:
- 1913
Licence: In copyright
Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.
Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.
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![THE MICIIOTOMIST'S VADE-MECUM. CHAPTER I. INTllODOOTOBY. 1. The General Method—The methods of modern microscopic anatomy may be rouglily classed as General and Special. There is a General or Normal method which consists in care- fully fixing the structures to be examined, staining them with a nuclear stain, dehydrating with alcohol, and mounting- series ('/ sections of the structures in halsam. It is by this method that the work is blocked out and very often finished. Special points are then studied, if necessary, by Special Methods, such as examination of the living tissue elements, in situ or in '''indifferent media; fixation with special fixing agents ; staining with special stains ; dissociation by teasing or maceration ; injection ; impregnation ; and the like. There is a further distinction which may be made, and which may help to simplify matters. The processes of the preparation of tissues may be divided into two stages. Pre- liminary Preparation and Ulterior Preparation. Now the processes of preliminary preparation are essentially identical in all the methods, essential divergences being only found in the details of ulterior preparation. By preliminary prepara- tion is meant that group of processes whose object it is to get the tissues into a fit state for passing unharmed through all the ulterior processes to which it may be desired to submit them. It comprehends the operations of (1) killing; (2) fixing ; [S] the washing and other manipulations necessary for removing the fixing agent from the tissues, and substi-](https://iiif.wellcomecollection.org/image/b21506802_0015.jp2/full/800%2C/0/default.jpg)
