The handling of chromosomes / by C.D. Darlington and L.F. La Cour.

  • C. D. Darlington
Date:
[1960], ©1960
Catalogue details

Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: The handling of chromosomes / by C.D. Darlington and L.F. La Cour. Source: Wellcome Collection.

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    APPENDICES ScH. I'.—RAPID DIOXAN METHOD FOR ROOT TIPS (La Cour 1937). A. Fixing: 12-24 hours in 10 c.c. glass phial (aqueous fixation). B. Washing: 3 quick changes of water in phial, C. Dehydration: In water-dioxan mixtures by steps. 25%, 50%, 75%, 2 hours in each. 100% dioxan overnight. E. Infiltration: Place directly in oven, adding wax of low M.P. at intervals for 4 hours. Transfer to pure molten wax for 2 hours. Sch. I .—SOFTENING METHOD FOR ANIMAL EGGS (after S. G. Smith 1940). A. Fixation: Kahle's Fluid without water, 2 hours (for Feulgen staining). [B. No washing.] С and D. Dehydration: By stages as follows:— follow by two changes of 4% phenol in я-butyl alcohol. E. Infiltration: Place directly in oven with equal amount of paraffin wax. Change to fresh molten wax after about 16 hours. F. Embedding: As usual. In preparing for sectioning cut down the block. Expose one side of the material and soak the block for 24 hours or more in water before cutting. A softening medium consisting of 9 parts 60% ethanol, i part glycerin, is recommended by Baker (1941).