Immunisation of animals against infection by Vibrion septique ann Bacillus chauvœi / by H.R. Allen and T.J. Bosworth.

  • Hubert Raymond Allen
Date:
[1924]
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Licence: In copyright

Credit: Immunisation of animals against infection by Vibrion septique ann Bacillus chauvœi / by H.R. Allen and T.J. Bosworth. Source: Wellcome Collection.

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    degree of immunity after two doses had been given, we were com- nelled to abandon this method as a practical measure owing to the serious local effects caused by the administration. We were obliged therefore to obviate these effects either by using a mixture of toxin and antitoxin, or by so modifying the toxin as to reduce its toxicity to a minimum whilst at the same time preserving its immunising properties. Both of these methods proved successful. Table 6.—Immunisation of Guinea-Pigs with Single Dose of “Vibrion Septique'' Mixture (50 Per Cent. Under- neutralised). Guinea-Pig. Inoculated, 23/10/23. Tested, 13/11/23. Result. “ V. Septique ” “ V. Septique ” Mixture. Toxin I. V. A (0-02 c.c. antitoxin 2 M.L.D. Lived. -j- 2 c.c. toxin) (0* 1 c.c.) B (0-02 c.c. antitoxin 2 M.L.D. Lived. + 2 c.c. toxin) (0-1 c.c.) C (0*02 c.c. antitoxin 2 M.L.D. Died 48 hours. + 2 c.c. toxin) (0-1 c.c.) D (0-02 c.c. antitoxin 5 M.L.D. Died 16 mins. -f- 2 c.c. toxin) (0-25 c.c.i E (0-02 c.c. antitoxin 5 M.L.D. Died 1 hour (approx.). -j- 2 c.c. toxin) (0-25 c.c.) Controls. F Nil 2 M.L.D. (0-1 c.c.i Died overnight. G Nil 2 M.L.D. (0 • 1 c.c.) Died 5 hours. H Nil 5 M.L.D. (0-25 c.c.i Died 13 mins. As was to be expected, we found that a double dose gave a much stronger immunity than a single one. A comparison of Tables 6 and 7 illustrates this point in the case of a toxin antitoxin mixture. From these it will be seen that we employed two methods as a test of immunity : (a) The intramuscular injection of culture and (b) the intravenous injection of toxin. We have found the intravenous injection of toxin to be just as reliable as a dose of culture in testing immunity, and we prefer its use, since the strength of the amount given can be much more accurately measured than is the case with culture. When two doses are given the first may consist of a toxin anti-
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