Histology; normal and morbid.
- Dunham, Edward K. (Edward Kellogg), 1860-1922
- Date:
- 1898
Licence: Public Domain Mark
Credit: Histology; normal and morbid. Source: Wellcome Collection.
Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.
415/466 page 419
![1 gram. 10 cc. 20 grams, 200 cc. METHODS OF STAINING. 419 toxylin, l)iit that devised Ijy Bohmer will iiiiswcr all purposes, and is verj' simple : 1. I Iiematoxylin crystals, Absolute alcohol, 2. Alum, Distilled water, Cover the solutions and allow them to stand over night. The next day mix them and allow the mixture to stand for one week in a wide-mouthed bottle lightly plugged with cotton. Then filter into a bottle provided with a good cork. The solution is then ready for use. Nearly all solutions of alum-hfematoxylin require an interval of time for ripening, and their staining-powers improve with age. Alum-htematoxylin is intended for staining sections from tissues that have been fixed and hardened. It is especially useful when the fixing-solution employed contained chromates, but may be used after almost any method of fixation, if the time of staining is of the right length and the sections are previously freed from acidity by thorough washing. If the following directions are closely adhered to, the student can hardly fail to obtain good results in the use of Bohmer's hfematoxylin: Transfer the sections from the 80 per cent, alcohol in which they have been kept to a dish of distilled water. At first thev vvill float on the surface of the water ; this is a favorable moment for removing all folds and wrinkles. The sections should be manipulated with ]ilatinum needles, prepared by fusing a bit of platinum wire into the end of a glass rod. Such needles can be cleaned by heating the wire red in a flame. When the sections sink to the bottom of the dish of water, and remain there, it may be assumed that they are free from alcohol. Filter about 5 cc. of the h?ematoxvlin into a watch-g-lass or butter- dish and transfer the sections from the water to the dye. Let the sections stain for three minutes by the watch, and then transfer them to a dish of distilled water. At first the sections will have a reddish tint, but as the washing proceeds the color will turn to a pure blue. During the washing the water should be renewed, until finally it acquires no color from the sections and the latter](https://iiif.wellcomecollection.org/image/b21223841_0415.jp2/full/800%2C/0/default.jpg)
