The content of sugar in the blood under common laboratory conditions / by Ernest Lyman Scott.

  • Scott, E. L. (Ernest Lyman), 1877-1966.
Date:
[1914]
Catalogue details

Licence: Public Domain Mark

Credit: The content of sugar in the blood under common laboratory conditions / by Ernest Lyman Scott. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

    7/50 page 273
    theless thought desirable to correlate the results for a single animal and by a single uniform method. In part because there has been comparatively little work done upon the cat, and in part for the reasons given below, this animal was selected for the research. Long ago Boehm and Hoffman called attention to some of the advantages of cats for laboratory work. They mentioned especially their cleanly habits, their uniform size and the fact that they had found them to be more uniformly healthy than other common animals available for estimation of the sugar of the blood. There are, however, other and perhaps more fundamental advantages. A large amount of work has been done on excised muscles — a type of experiment for which the cat seems to be particularly adapted. Notes published by Lee and by Lee and Harrold show that some of this is directly related to the use of sugar by the organism. Again, some authors, as Macleod and Pearce, have sought to avoid, by decerebration, the extended use of drugs in experiments where prolonged anesthesia is necessary. The same results may be obtained in a more physiological manner, and with less hemorrhage, by cerebral anaemia. Leonard Hill and Stewart with his co-workers have shown that the dog, because of peculiar- ities of the blood-supply to the brain, is not so well adapted for this procedure as is the cat. Pike has confirmed Porter's state- ments that cats are better adapted for experiments involving vaso- motor responses than are dogs. II. —■ Method of Analysis The preparation of the animal and method of obtaining the blood will be discussed later. Only the chemical processes in- volved will be described here. It is not possible to estimate the sugar by any known method in the presence of protein. Many reagents have been used and many methods proposed for the re- moval of the protein from blood preparatory to the determination of sugar. In 1908 ]\IichaeHs and Rona ^ proposed the use of colloidal iron hydroxide for this purpose. Their method has been well received and is widely used at present. Recently, however, Lesser reports that it is not satisfactory, in the form proposed by the authors for the blood of either frogs or turtles. In the
    digitised image 1
    digitised image 2
    digitised image 3