Playing God : genetic engineering and the manipulation of life / June Goodfield.
- June Goodfield
- Date:
- 1977
Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Credit: Playing God : genetic engineering and the manipulation of life / June Goodfield. Source: Wellcome Collection.
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![Appendix I: The NIH Guidelines The guidelines define four levels of physical containment, designated, in order of increasing stringency, Pi to P4, and three levels of biological con¬ tainment, EKl to EK3, and assign exf)eriments to them on the basis of potential risk. The following is a summary of containment levels specified for various sources of DNA. a. Shotgun experiments using E. coli as the host Non-embryonic primate tissue Embryonic primate tissue or germ line cells Other mammals Birds Cold-blooded vertebrates, non-embryonic embryonic or germ line If vertebrate produces a toxin Other cold-blooded animals and lower eukaryotes If Class 2 pathogen,* produces a toxin, or carries a pathogen Plants Prokaryotes that exchange genes with E. coli Class 1 agents (non-pathogens) Low-risk pathogens (for example, enterobac- teria) Moderate-risk pathogens (for example, S. typhi] Higher-risk pathogens Prokaryotes that do not exchange genes with E. coli Class 1 agents P3+EK3 or P4+EK2 P3+EK2 P3+EK2 P3-I-EK2 P2+EK2 P2+EK1 P3+EK2 P2+EK1 P3+EK2 P2+EK1 Pl+EKl P2-I-EK1 P2-I-EK2 banned P2+EK2 or P3+EK1 'Classes for pathogenic agents as defined by the Center for Disease Control.](https://iiif.wellcomecollection.org/image/b18032060_0231.JP2/full/800%2C/0/default.jpg)
