Affinity labelling and cloning of steroid and thyroid hormone receptors / edited by H. Gronemeyer.

Date:
[1988]
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Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Affinity labelling and cloning of steroid and thyroid hormone receptors / edited by H. Gronemeyer. Source: Wellcome Collection.

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    Ch. 17] Characterization of a c-erbA-thyroid hormone receptor cDNA 311 specific effects of \-erbA in erythroblasts have been observed: it completely blocks the spontaneous differentiation exhibited by transformed erythroblasts, and it allows cell proliferation at pH and ionic conditions otherwise toxic to the cells (Kahn et al., 1986b; Damm et al., 1987). The effects of \-erbA in fibroblasts are less pronounced, but changes in cell тофЬо1о§у and enhancement of \-erbB induced focus formation have been observed (Frykberg et al., 1983; Jansson et al., 1987). IL EXPRESSION OF THE с-егЬЛ GENE То understand better the oncogenic effects of \-erbA, an analysis of the expression of the c-erbA gene by the Northern procedure was done. The results revealed that two RNAs, 4.5 and 3.0 kb in length, were found in many cell types (Fig. 1), and that Fig. 1 — Expression of the 4.5 and 3.0 kb c-erbA mRNAs in chicken and quail tissues. Polyadenylated RNA, isolated from 10-day-old embryos or from tissues of 3-week-old chicks, was separated on denaturing gels, transferred to filter paper and hybridized with a probe recognizing both c-erbA and c-erbB (12 and 9 kb) RNAs. 25 ¡ig RNA was analyzed unless indicated otherwise. additional erbA related RNAs could be detected in bone marrow cells. Whether or not these RNAs derive from the same c-erbA locus as the 4.5 and 3.0 kb RNAs is unclear. Additional experiments have shown that the 4.5 and 3.0 kb RNAs are