Affinity labelling and cloning of steroid and thyroid hormone receptors / edited by H. Gronemeyer.
- Date:
- [1988]
Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Credit: Affinity labelling and cloning of steroid and thyroid hormone receptors / edited by H. Gronemeyer. Source: Wellcome Collection.
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![Index 319 peptide maps of A and B, 171-174 photoaffinity labeling of, 55-59, 166-182 equipment for, 58-59 identification, 168-174 protocol, 59 receptor crosslinking in cytosol, 170-171 purification, 59-63 classical chromatography, 60-62 generation of antibodies, 63 methodologies of, 59-60 preparation of cytosol, 60 preparative SDS-gel electrophoresis, 62-63 structural analysis, 63-66 amino acid sequence analysis, 66 column supports, 66 partial proteolytic maps, 63, 65 peptide isolation and sequencing, 65-66 S-Cm-, form B, separation of tryptic peptides, 179, 180-181 chloramphenicol acetyl transferase (CAT), 216 llß-chloromethylestradiol, 90 chymotrypsin, 49, 51, 160,178 a-, 63 'Cleveland maps', 171 of chicken progesterone receptor, 172 Cortisol, electrophilic affinity labeling by, 37 Cortisol 21-mesylate (CM), 111 preparation and structure of, 30-31,110 cosmid cloning vectors, 248 8-CPT-c AMP, 116 crosshybridization studies, 245 cytosol receptors labeling of, 20 partial purification of, 24 deacylcortivazol 21-mesylate (DAC-Mes) electrophilic affinity labeling of cell-free non¬ receptor molecules by, 36-37 preparation and structure of, 30, 31 desmethylnafoxidine aziridine (Naf-Az), 18,19, 90,91,92 dexamethasone, 31, 131, 145, 146 electrophilic affinity labehng of protein by, 37 [^H], binding to DNA-cellulose pellets, AA-Al dexamethasone 21-mesylate (DEX-Mes), 111, 131 affinity labeling of cell-free receptors by, 32-34 antagonist activity of, 115-116 covalent labehng of previously bound receptors, 34 electrophilic affinity labehng of cell-free non¬ receptor molecules by, 36-37 exchange binding, 34 mechanism of irreversible antiglucocorticoid action of, 116-119 preparation and structure, 30, 110 pH]dexamethasone 21-mesylate, 37, 38, 39 bound receptors, binding to DNA-cellulose pellets, 44-47 labeled receptors activation of, 42-43 'double DNA-cellulose chromatography' purification of, 43-44 immunoadsorption of, 41-42 localization of modified amino acids in, 49-52 (NH4)2S04 precipitation of, 41 pi values, 131 proteolysis of unactivated and activated, 48-49 dextran-coated charcoal assay, 200 DHT-bromoacetate, protocol for synthesis of, 77 ['H], 76 N-2-diazo-3,3,3-trifluoropropionyl-L- triiokdothyronine (L-T -PAL), 213, 214 regulation of receptor levels mechanism of, 221 21-diazodexamethasone, 31-32 [^H]dihydrotestosterone, 200 dihydrotestosterone 17ß-bromoacetate, 78, 206 structure, 203 synthesis of, 203 [^HJdihydrotestosterone 17ß-bromoacetate binding to androgen receptor, binding affinity, 203-204 covalent attachment, 204-206 steroid specificity, 204 17a,21-dimethyl-19-nor-pregna-4,9-diene-3,20- dione (R5020), 35, 36, 55, 56, 60, 61, 71, 168 absorption spectrum of, 68, 69 crosslinking with, 56 PH]-, 59 photocrosslinking to progesterone receptor, 69-71 structure, 31, 32,169 DNA binding domain, 269-271 -binding finger, 249 competition binding assay, 47, 48 filter binding and footprinting, 47-48 transilluiminators, 68 cDNA cloning, 253-257 strategies for receptor, 244-248 system, towards a powerful, 241-244 cDNAs, deletion mutations introduced into receptor, 155-156 'double DNA-cellulose chromatography', 43-44 'doublet B-singlet A', 189-191 electrophilic affinity labeling, 29 labels, 30-31 specificity of, 120 endocrine therapy, breast cancer, 89 'enhancers', 268 Escherichia coli hfl, 242 estradiol, 90 ['H], 22 estrogen receptor affinity labeling agents for, 18-20 applications of, 97-104 conditions for, 94-97 systems, 90-97](https://iiif.wellcomecollection.org/image/b18029310_0324.JP2/full/800%2C/0/default.jpg)
