Affinity labelling and cloning of steroid and thyroid hormone receptors / edited by H. Gronemeyer.

Date:
[1988]
Catalogue details

Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Affinity labelling and cloning of steroid and thyroid hormone receptors / edited by H. Gronemeyer. Source: Wellcome Collection.

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    Index 321 human glucocorticoid receptor cDNA cloning, 257 gene promoter, 298-307 human lymphoblastic leukaemia cell line CEM- C7,147 human progesterone receptor, 68-74 A and В form independent 8S haloreceptors, 191-193 A and В hormone-binding proteins, 195-197 covalent modifications in the nucleus of, 192-195,196 'doublet B-singlet A', 189-191 optimization of photolysis conditions, 68-69 phosphorylation of A and В in nuclei, 194 photoaffinity labeling of, 186-197 in situ, 71-72 in vitro, 69-71 representativeness of, 187 R5020 photolysis and cellular UV damage, 187-189 SDS-polyacrylamide gel electrophoresis, 72-74 human T47D receptor, 168 human T47D system, 56 transformation reaction, 193, 194 ß-hydroxy-a-keto mesylate, 31 hydroxylapatite batch assay, 200 IgG,41,42 2-iminothiolane, 24, 25, 103 intact cells, labeling of, 21-22 iododesethyltamoxifen aziridine (I-Tam-Az), 91, 92 ^^^I-iodothyronine analogues, 227-230 irreversible antiglucocorticoids activity, 111-114 affinity labels, and. 111 isoelectric focusing, 8M urea, 23 ketononestrol aziridine (KN-Az), 18,19, 91, 92 bioactivity of, 97 PH],21,22 Д^-ketosteroid isomerase, 56 leupeptin, 20 ligand affinity chromatography, 59,174-175 MCF-7 cells, 25, 88, 99,116, 257 cytosol preparation, 21 b-mercaptoethanol, 25, 42 'mero' receptor, 171,172, 173 methanesulfonate, 18,19 methyltrienolone (R1881), 206, 208 pH], 200 in labeling androgen receptor, 209 [^H]Mibolerone, 200 molybdate, 20, 60 mouse lymphoma cells, receptor genotypes of, 157-159 MMTVRNA levels, in MMTV infected Fu5-5 cells, 116 Mr receptor forms, 224-225 half-life and relative synthetic rates of, 226-227 murine thymic lymphoma line S49 1 cells glucocorticoid effect on cloning, 146 growth,145-146 murine thymic lymphoma WEHI-7, 147,151 nafoxidine, 90, 91 p-nitrobenzylpyridine (pNBP), 31 non-equilibrium pH gradient electrophoresis (NEPHGE), 39, 130 Northern procedure, 311 nuclear estrogen receptor, chemical crosslinking of, 24-25 nuclear receptor extracts, preparation of, 21-22 nuclear receptor family, 252-290 nuclear receptors, partial purification of, 24 Okayama-Berg cloning technique, 242, 243 ORG 2058, structure of, 169 ovalbumin (3.55), 24 3-oxo-4-benzyl-17ß-acetoxy-andostane, 57 6-oxo-estradiol, 57 3-oxo-4-estrene-17ß-ylacetate, 56 PAL derivatives, synthesis and purification of, 81 phenylmethylsulfonyl fluoride (PMSF), 20, 60 photoaddition reaction, 57 photoaffinity labeling, 29 cell-free cultures, 34-36 labels, 31-32 specificity of, 120 see also under individual headings PR-6, 191,192 promegestone, 150 progesterone receptor A-B, 168 chicken, see chicken progesterone receptor functional domains of, 283-287 human, see human progesterone receptor 'molybdate-stabilized' form of, 175 rat glucocorticoid receptor, structure of, 121-122 hepatoma tissue cultured (HTC), 111 covalent labehng of, 33-34 preparation of, 32-33 Uver receptors covalent labeling of, 34 preparation of, 33 serum albumin (RSA), labeling of, 36 uterine cells, pulse-chase experiments in vitro of, 25-26 uterine estrogen induced protein, 88 uterine estrogen receptor, labeling of, 20-21 receptor heterogeneity, affinity labeling of, 127-131 receptors 'increased nuclear transfer', 148 'nuclear transfer deficient', 148 see also under individual headings retinoic acid receptors, chromosomal localization of, 270 RNA, 132