Immunoflourescence: Trypanosome brucei brucei

Life cycle of sleeping sickness causing parasite This is a high power immunofluorescence micrograph of a Trypanosoma brucei brucei parasite (procyclic form), from the midgut of a tsetse fly. This trypanosome was stained with an antibody against a paraflagellar rod protein to show the flagellum in green, and with a stain to show the DNA present in the nucleus and kinetoplast in blue. Procyclic forms are found in the midgut of the tsetse fly. They develop from the short-stumpy trypanosomes taken up with the blood meal from an infected mammal.