Chemical examination of the root of ipomoea orizabensis / by Frederick B. Power and Harold Rogerson.

  • Frederick Belding Power
Date:
[1912?]
Catalogue details

Licence: In copyright

Credit: Chemical examination of the root of ipomoea orizabensis / by Frederick B. Power and Harold Rogerson. Source: Wellcome Collection.

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    purified sawdust, and the thoroughly dried mixture then succes¬ sively extracted in a large Soxhlet apparatus with the above- mentioned solvents. I.—Petroleum Extract of the Resin. This extract, after the removal of the solvent, was a dark brown, soft solid, amounting to 71 grams. It was dissolved in ether, and the ethereal solution extracted successively with aqueous ammonium carbonate and potassium carbonate, which, however, removed nothing. A subsequent extraction with a solution of sodium hydroxide removed only a very small amount of fatty material, which could not be obtained crystalline, and a trace of a yellow substance which gave a bright red colour with both acids and alkalis. The ethereal liquid was then washed with a little water, dried, and the solvent removed, when a quantity of a soft solid remained. This was dissolved in alcohol, and heated for about four hours in a reflux apparatus with an excess of potassium hydroxide, after which the mixture was diluted with water, and the alcohol removed in a current of steam. The alkaline liquid was then thoroughly extracted with ether, the ethereal liquid being washed, dried, and the solvent removed, when a crystalline residue was obtained. Isolation of IIentriacontarie, C31HC4, and a Phijtosterol, C07H460. The above mentioned crystalline residue was dissolved in warm alcohol, when, on cooling, a small amount of a solid was deposited, which was collected and recrystallised from ethyl acetate. Two fractions were thus obtained, the first being very small in amount and melting at 60—62°, whilst the second fraction separated in plates, melting at 133—135°. The first fraction gave the colour reaction of the phytosterols, and it was therefore heated for a short time with a little phthalic anhydride. After this treatment there was isolated from the mixture a small amount of a hydro¬ carbon, which separated from ethyl acetate in leaflets melting at 6G—68'This substance was evidently hentriacontane, C3]HG4 (m. p. 68°), and when mixed with a little of this hydrocarbon of known purity the melting point remained unchanged. i he alcoholic liquid from which the above-mentioned solid had separated was concentrated, when a further quantity (IT grams) of substance was deposited in the form of leaflets. This was mixed with the previously obtained fraction (0‘2 gram) melting at 133—135°, and the whole recrystallised from a mixture of ethyl