The elements of bacteriological technique : a laboratory guide for medical, dental, and technical students / by J.W.H. Eyre.

  • Eyre, John W. H. (John William Henry), 1869-
Date:
1913
Catalogue details

Licence: In copyright

Credit: The elements of bacteriological technique : a laboratory guide for medical, dental, and technical students / by J.W.H. Eyre. Source: Wellcome Collection.

    102/564
    (d) Thionin blue. (e) Bismarck brown. (f) Neutral red. (B) Acid: (a) Eosin, aqueous yellowish. (b) Safranine. These dyes are kept in i per cent, aqueous solution to which is added ^ per cent, of alcohol, as a preserv'a- tive. They are generally used in this form. A few nuclear stains (carmine, hiematoxylin) are occasionally used more especially in “section” work. Decolotirisaiion.—After overstaining, films may be decolourised by washing for a longer or shorter time in one of the following reagents arranged in ascending order of power 1. Water. 2. Chloroform. 3. Acetic acid, i per cent. 4. Alcohol. 5. Alcohol absolute. Acetic acid, i percent., j Hydrochloric, i per cent, aqueous solution. Hydrochloric, i per cent, alco- holic (90 per cent.) solution. } equal parts. 6. Mineral acids: Sulphuric, 25 per cent, aqueous solution. Nitric, 33 per cent, aqueous solu- tion. Counter staining.—Use colours which will contrast with the first stain; e. g., Vesuvin, for films stained by methylene-blue or Gram’s method. red, Neutral Eosin, Fuchsin, Methylene-blue, Gentian violet. ] for films stained by fuchsin. /