Ferment actions of the pancreas in different animals / by Vincent D. Harris and William J. Gow.

  • Vincent Dormer Harris
Date:
1892
Catalogue details

Licence: Public Domain Mark

Credit: Ferment actions of the pancreas in different animals / by Vincent D. Harris and William J. Gow. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Experiment III. Raw starch grain ^ + solution of HCl 1 50 c.c. Placed in incubator at a temperature of 90® F. for 24 hours. No sugar reaction. After boiling, cooling and adding iodine to some of the solution, a pure blue colour appeared. The acidity of the solution was neutralized and rendered alkaline to the extent of 1 Sod. Garb. Some fresh pig’s pancreas previously pounded in a mortar was added and the mixture was replaced in an incubator for 24 hours. On again testing the mixture there was no evidence of the presence of sugar. After boiling, and cooling, iodine solution was added. A reddish colour appeared which rapidly disappeared. Fresh pig’s pancreas appears therefore to have the power of converting raw starch into dextrin. Effect of Antiseptics on Starch Digestion. Two flasks were taken. A. 50 c.c. (1 ®/g Starch solution). 1 ®/o Sod. Garb. 5 c.c. of Brine extr. (Ox). 10 c.c. water. £. 50 c.c. (1 ®/„ Starch solution). 1 ®/„ Sod. Garb. 5 c.c. of Brine extr. (Ox). 10 c.c. of 5 ®/^ Solution of Acid Garbol. Proportion of Acid Garbol. = 1—130. Placed in incubator. At end of 24 hours faint reddish colour in both flasks, on adding iodine. Sugar estimated by Pavy’s method shewed an equal quantity in both flasks. A. 50 c.c. Starch solution (1 ®/^) + 1 ®/^ Sod. Garb. + 5 c.c. Brine Pig’s. £. 50 c.c. Starch solution (1 ®/^) + 1 ®/^ Sod. Garb. 5 c.c. Pig’s + Hyd. Perchlor. gr. 1 /20. ' Incubator 24 hours. Sugar estimated and found equal in both flasks, thei-efore conversion of starch into sugar not hindered by presence of acid carbol. 1 in 130 of Hyd. Perchlor. 1—1000. These and similar experiments, with reference to the other ferments proved conclusively that antiseptics do not interfere with the action of the unorganized ferments of the pancreas and confirm the results of former experiments by one of us, reported in a former volume of this JoumaD. * Harris and Tooth. Vol. ix. p. 220.
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