Anærobic infection in animals : discussion on Professor Gaiger's paper Mr. Dalling's views.

  • Dalling, Thomas.
Date:
[1924?]
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Licence: In copyright

Credit: Anærobic infection in animals : discussion on Professor Gaiger's paper Mr. Dalling's views. Source: Wellcome Collection.

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    It was found that the use of a pure toxic filtrate produced necrotic lesions in guinea-pigs and marked local oedema in sheep on inoculation, hence a mixture of toxin and antitoxin was used for immunising purposes—a 25 per cent, under neutralised toxin giving good results. Table V. shows how such neutralisation is carried out. The smallest dose of antitoxin that will protect against a certain lethal dose of the toxin is taken as the neutralising dose of toxin. By calculation the amount of antitoxin required to neutralise the amount in a given batch of toxin is ascertained. 25 per cent, of this amount is added to the batch. Table V. Mouse. Toxin. Anti-toxin. Result. A •5 c.c. + 3 mins. B ‘5 c.c. + 5 mins. C •25 c.c. + 5 mins. D •25 c.c. + 7 mins. E •25 c.c. •003 c.c. + 3 mins. F •5 c.c. •006 c.c. + 6 mins. G •5 c.c. •012 c.c. + o.n. H •5 c.c. •025 c.c. •— J •5 c.c. •05 c.c. — K •5 c.c. 1 c.c. -- It has also been ascertained that the filtrate from a forty-eight hour culture is of good antigenic value. Protective Value of Mixture in Guinea-pigs. Table YI. shows that one inoculation of a Vibrion septique mixture produces a certain degree of immunity only, while Table VII. shows that a double inoculation adds greatly to the amount of immunity produced. Table VI. Guinea-pig. V.S. Mixture. V.S. Toxin Result. (Intravenously). A 2 c.c. •25 c.c. Lived. B 2 c.c. •25 c.c. Lived. C 2 c.c. •25 c.c. Died 48 hrs. D 2 c.c. •25 c.c. Died 1 hr. E •1 c.c. Died 5 hrs. F •1 c.c. Died 13 mins. Toxin injected a fortnight after mixture.
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