Molecular approaches to eucaryotic genetic systems / edited by Gary Wilcox, John Abelson, C. Fred Fox.

Date:
1977
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Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Molecular approaches to eucaryotic genetic systems / edited by Gary Wilcox, John Abelson, C. Fred Fox. Source: Wellcome Collection.

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    2 1. JOHN С. FIDDES origin of viral strand DNA synthesis are known (17,18). The nucleotide sequence of 0X174 would thus be expected to provide information about the overall organisation of the phage genome and of the nature of these control sites. Largely through use of the rapid plus and minus technique an almost complete nucleotide sequence has now been obtained by Sanger et al. (1) for the 5375 nucleotides of 0X174. In this paper it is intended to discuss some features of this sequence, RESULTS AND DISCUSSION 0X174 NUCLEOTIDE SEQUENCE Coding sequences. Genes D, J, F, G and H of 0X174 are transcribed from a promoter preceding gene D (see Fig. 1). Fig. 1. Genetic map of bacteriophage 0X174, based on the data of Sanger et al. (1). The 0X174 genes A, A, B, C, D, E, F, G, H and J are indicated along with the size of the intercistronic spaces, the locations of the transcriptional starts and the origin of viral strand DNA synthesis. The inner circle represents the fragments produced by the enzyme Hae III (Z).