Practical bacteriology for chemical students / by David Ellis.

  • David Ellis
Date:
1923
Catalogue details

Licence: In copyright

Credit: Practical bacteriology for chemical students / by David Ellis. Source: Wellcome Collection.

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    CONTENTS. CHAPTER PAGE I. Bacteriological apparatus, Steam-steriliser: Hot-air oven: Autoclave: Incubator: Small apparatus requir- ed by each student: Hints to students i II. Preparation of Nutrient-media. Sterilisation of Ap- paratus 14 III. Agar-plate culture: Agar-slope culture: Gelatine-plate culture: Gelatine stab culture: Gelatine shake culture: broth culture. Revision 23 IV. The Staining of Bacteria. Use of Aniline dyes. Pre- paration of Stains. Dry-film staining. Wet Staining. Irrigation dry-film method. Hanging-drop preparation 34 V. Identification and Staining of Spores. Staining of cilia. Germination of Spores. Capsule staining. Contact preparation 42 VI. Reserve food materials. Volutin, glycogen and oil- globules. Gas production. Acid production. Indol production. Milk cultures 52 VII. Anaerobic bacteria. Methods of plating and of cul- tivating anaerobic bacteria. Methods of estimating the size of bacteria and of other minute objects. Production of H2S in bacteria. Milk Cultures . . 63 VIII. The Isolation of bacteria from soil, from hay-infu- sion and from air. Counting of Colonies. Estima- tion of rate of movement of bacteria. Medium for cultivation of chromogenic bacteria 78 IX. Examination of Drinking Water. Identification of Bac. coli communis. Total count of bacteria in drinking water. Methods of search for Bac. enteri- tidis sporogenes 86