Practical bacteriology for chemical students / by David Ellis.

  • David Ellis
Date:
1923
Catalogue details

Licence: In copyright

Credit: Practical bacteriology for chemical students / by David Ellis. Source: Wellcome Collection.

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    (c) The spores, which are enclosed in a sporangium (see Figs 44, 45). Compare their size with that of the spores of bacteria and of the Yeast cell. Exercise 104. Examination of a green mould. 1. Examine in the same way as in the preceding exercise. Observe (a) The hyphae which form numerous branches. (b) The presence of transverse walls. (c) The spores, which arise in small clusters of chains. The hyphae in each cluster which give rise to the spores arise all from a small upright hypha which is attached to the main body of the hyphae. Exercise 105. Germination of Mould-spores. 1. Construct a germination-chamber in the manner indicated in Exercise 99, using for preference the glass-ring described in the note attached to that exercise (see Fig- 47)- 2. Examine a Mould-culture to see if spores are developed in abundance. 3. If spores are present in abundance, rub a sterile moistened Plat, loop in the mass of the mould so that when withdrawn it cannot fail to remove with it a number of spores. 4. Place a drop of melted wort-gelatine on a cover-slip: the latter must be large enough to cover the glass-ring. 5. Rub the Plat, loop in the wort-gelatine so that some of the spores are transferred from the loop to the wort- gelatine. 6. With a sterilised Plat, loop rub the wort-gelatine over the surface of the coverslip until it sets in the form of a film. 7. Place a drop of water at the bottom of the glass-ring, on the slide, then close up the cavity formed by the glass- ring by placing the coverslip on top of it. In doing so, take care that the film side of the cover-slip is on the under-side.