Practical bacteriology for chemical students / by David Ellis.

  • David Ellis
Date:
1923
Catalogue details

Licence: In copyright

Credit: Practical bacteriology for chemical students / by David Ellis. Source: Wellcome Collection.

    133/152 (page 121)
    to the spore any time by focussing within the black circle when the spore will be found without trouble. 3. Draw about -t dozen of these circles on the cover-slip, each one of course circling a spore which it is desired to investigate. If any difficulty is experienced in making circles that are not splotchy on the glass, the difficulty can be remedied by taking precautions to make the upper side of the cover-slip absolutely clean and dry. Method II for location of spores. Instead of using an ordinary coverslip for the experiment, the squared slide may be employed (See Exercise 96). Note on Mucor mucedo. The spores inside the sporangia are asexual and cor- respond to those spores of Penicillium that are normally so abundantly formed. Like Penicillium Mucor also forms sexual spores but for our purpose their importance is not great. The fact must be noted with care that whilst the asexual spores of Mucor are formed inside a spore-box or sporangium, the similar spores of Penicillium are formed by abstriction from the ends of hyphae. Exercise 106. To make a pure culture of Mucor mucedo. 1. Into a sterile small test-tube pour about an inch of sterile water. 2. With a sterilised Plat, loop pick up a tiny portion of the Mucor culture and mix this material with the water in the test-tube. 3. Sterilise loop and lay it on the rest. 4. Melt a tube containing the nutrient medium the prepara- tion of which is described in Exercise 107, Section A. 5. Pick up loop and sterilise it. 6. With loop remove a loopful of infected water from test-tube.