Practical bacteriology for chemical students / by David Ellis.

  • David Ellis
Date:
1923
Catalogue details

Licence: In copyright

Credit: Practical bacteriology for chemical students / by David Ellis. Source: Wellcome Collection.

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    8. Shake thoroughly—but gently—to establish a good distribution of the infected water. 9. Pour contents into sterile Petri-dish. 10. Set aside, well covered, at room temperature until colonies appear. 3i. When colonies have appeared, touch one which is well apart from the other colonies with a sterilised Plat, needle. 12. Then stab this Platinum needle into a tube containing the same nutrient medium as was used above for Petri- dish culture. Exercise 107. Media for cultivation of Fungi. A. Maltose-peptone Agar. Maltose 4 grams Peptone 1 gram Agar 1-5 grams Water 100 c.c. Normal hydrochloric acid 2 c.c. B. Maltose-peptone broth. Same as A but with the omission of Agar. C. Plum-gelatine. Boil 100 grams of dried plums in 100 c.c. water. Make up to 200 c.c. then add 20 grams gelatine. D. Bread-mash. Add enough water to dry crumbled bread to make a mash. Sterilise in steam steriliser. E. Rice-mask. Rice meal 10 grams milk 15 c-c- maltose-peptone broth (B) F. Beer-wort Agar. Beer-wort Agar . 100 c.c. iF grams. Identification of the Commoner Moulds. Mucor mucedo — Found in excreta of herbivorous animals, particularly in horse-excreta. Sporangia as in Fig. 44.