The testing and standardization of B. welchii (perfringens) antitoxin / T. Dalling [and others].

Date:
[1928?]
Catalogue details

Licence: In copyright

Credit: The testing and standardization of B. welchii (perfringens) antitoxin / T. Dalling [and others]. Source: Wellcome Collection.

    4/10 page 44
    Table I.—Methods of Testing Toxin. Pigeon . Intramuscular Mouse. ,, „ . Intravenous Guinea-pig . Intracutaneous . M.B.D. In vitro . Haemolysin . M.H.D. . Bull, 1917 ; Bengtson, 1920. . Present authors, 1918— 1928 (M.R.C. Report, 1919). . O'Ol to 0T c.c. . Ditto. ,, ,, . Klose, 1916; Glenny and Allen, 1921. ,, ,, . Henry, 1917; Wein¬ berg, 1918. M.L.D. . 0T to 1‘0 c.c. Table II.—Titration of Standard Serum Against Fixed Doses of Toxin. In vitro. Mouse. Guinea-pig. Dose of standard serum. Haemolysis. s Intra¬ venous. Intra¬ muscular. Intradermic. 0T018 Complete • • • Died • • • 5 5 Necrosis. 0-0020 • J5 Died . GEdema . Slight necrosis ? ? Lived 0-0022 . Nearly complete . 55 • 5? No necrosis. Lived 5 > 00025 . Slight haemolysis . 5 5 • >> 0-003 No haemolysis ? ? >> 5 5 • 5 5 )> Table III.—Neutral Points. Standard serum 0*0025 0*0022 . 0-0020 . 0*002 Serum A 0014 0*012 . o-oi 0*012 „ B . 0-00065 0-0006 . 0-0006 . 0-00055-75 „ c . 0*0021 0*002 . 0-0017 . 0*0016 „ D . o-ooi 0-00075 . 0-0007 . 0*0008 Calculated Values. (Standard = 45 units.) Serum A 8 8 . 9 . 8 ,, B . 170 160 . 150 . 120-170 „ c . 50 50 50 . 60 ,, D . 110 130 . 130 120
    digitised image 1
    digitised image 2
    digitised image 3