Methods of pathological histology / by C. von Kahlden ; translated and edited by H. Morley Fletcher.

Date:
1894
Catalogue details

Licence: Public Domain Mark

Credit: Methods of pathological histology / by C. von Kahlden ; translated and edited by H. Morley Fletcher. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    recently-killed animal for twenty-four hours in a mixture of 5 per cent solution of potassium bichromate. 2 per cent solution of perosmic acicl. (2) Wash for several hours in running water. (3) Harden in 75 per cent, then 90 per cent, and lastly in absolute alcohol. (4) Saturate with xylol and embed in paraffin (vide p. 24). (5) Cut. (6) Eemove the paraffin with xylol from the section which is fixed to the slide, then wash with alcohol. (7) Place on it a few drops of a 20 per cent solution of acid- fuchsine in cold saturated aniline water. Warm over a flame until the fluid steams. (8) Allow it to cool, then wash away the staining fluid with a mixture of a concentrated alcoholic solution of picric acicl one part, with water two parts. (9) Pour on it some picric acicl solution, and carefully warm for thirty to sixty seconds on the paraffin oven. (10) Pass it through alcohol and then xylol. William Russell has described the following method for showing bodies occurring in the cells of tumours :— (1) Harden in alcohol or Midler's fluid. (2) Stain for ten minutes or longer in a saturated solution of fuchsine in a 2 per cent aqueous solution of carbolic acid. (3) Wash in water for a few minutes. (4) Wash in absolute alcohol for half a minute. (5) Stain for five minutes in a 1 per cent solution of iodine green in a 2 per cent carbolic solution. (6) Dehydrate quickly with absolute alcohol, oil of cloves, Canada balsam. [ATotc— In preparations which may require to be photographed under the micro- scope, the use of methylene blue should be avoided, as it is the worst stain which can be employed for this purpose.—Ed.] j- aa.
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