Chemical examination of the bark of Euonymus atropurpureus / by Harold Rogerson.

  • Rogerson, Harold.
Date:
1912
Catalogue details

Licence: In copyright

Credit: Chemical examination of the bark of Euonymus atropurpureus / by Harold Rogerson. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    sulphide for the removal of the lead, and, after filtering, was con- centrated under diminished pressure to the consistency of a syrup. This contained a quantity of sugar, since it readily reduced Fehling’s solution, and yielded d-phenylglucosazone melting at 208—209°. Examination of the Resin (B). This was a dark brown, soft solid, and amounted to about 730 grams, being thus equivalent to 3'2 per cent, of the drug. It was dissolved in alcohol, mixed with purified sawdust, and the thoroughly dried mixture successively extracted in a Soxhlet apparatus with light petroleum (b. p. 40—60°), ether, chloroform, ethyl acetate, and alcohol. Petroleum Extract of the Resin. This was a dark brown mass, which amounted to 460 grams. It was dissolved in ether, and the ethereal liquid extracted successively with aqueous ammonium and sodium carbonates, but nothing was removed by this treatment. The ethereal solution was therefore distilled, and the residue heated in alcoholic solution with an excess of potassium hydroxide. The alcohol was then removed, and, after cooling, water was added and the alkaline liquid thoroughly extracted with ether. The ethereal liquid was dried, and the solvent evaporated, when there remained a quantity (70 grams) of a viscid mass, which became solid on cooling. This was dissolved in a large volume of alcohol, when, on keeping for some days, a quantity (12 grams) of a crystalline product separated in flat needles. On recrystallising this product from a mixture of ethyl acetate and dilute alcohol, it separated in laminae melting at 135—136°, which gave colour reactions similar to those of the phytosterols. Isolation of a New Monohydnc Alcohol, Euonysterol, C3lIl5iO'OH. The above-described product was found to be a mixture, and, after fractional crystallisation, each fraction was converted into its acetyl derivative, and these recrystallised several times. In this way, small amounts of amorphous material were eliminated. The acetyl derivatives were then hydrolysed with alcoholic potassium hydroxide, and the resulting products recrystallised from a mixture of ethyl acetate and dilute alcohol. The principal fraction consisted of flat needles melting at 137—138°: 0'2494, on heating at 110°, lost 0‘0132 H20. H20 = 5'3. 0T166 * gave 0 3466 C02 and 0'1216 H20. C = 81*l; H = 11‘6. C3]H5202,1£H20 requires Ii20 = 5'6 per cent. C31H5202 requires C = 81‘5; H = 11'4 per cent. * Anhydrous substance.
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