The division and post-fission movements of bacilli when grown on solid media / by G.S. Graham-Smith.

  • George Stuart Graham-Smith
Date:
1910
Catalogue details

Licence: In copyright

Credit: The division and post-fission movements of bacilli when grown on solid media / by G.S. Graham-Smith. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

    3/50
    [From PARASITOLOGY, Vol. III. No. 1, 1910] [All Rights reserved] THE DIVISION AND POST-FISSION MOVEMENTS OF BACILLI WHEN GROWN ON SOLID MEDIA. By G. S. GRAHAM-SMITH, M.D. (University Lecturer in Hygiene, Cambridge). (Plates III—VIII and 14 Text-figures.) The behaviour of living bacteria during artificial cultivation in fluid and semi-solid media has often been observed and described, but very few investigators appear to have studied the changes which take place when bacteria are grown either on the surface or in the depth of solid media. The investigations which are described in this paper were undertaken with the purpose of ascertaining how various bacilli behaved during the early stages of growth on the surface and in the depth of agar, and how far the various types of division and post-fission move- ment influenced the characters of the colonies subsequently formed. Hill (1901) devised a method for watching the growth of bacteria on agar and carefully described the mode of growth of B. diphtheriae and of B. typhosus. His “ technique consists briefly in substituting for the ordiuary ‘ hanging drop ’ of liquid or jelly a cube of solidified agar, on the surface of which the bacteria are distributed. The inoculated surface of this cube is applied to the under surface of a cover-slip, and for convenience is known then as the ‘ hanging block.’ Oxygen probably reaches the bacteria by diffusion through the block or the seal. Certainly aerobic bacteria like B. diphtheriae, B. typhosus, etc., grow readily in such preparations.” Hill (1902, p. 204) gives the following directions for preparing hanging block preparations. “ Pour melted agar into a Petri dish to the depth of about one-eight to one-quarter inch. Cool this agar and cut from it a block about one-quarter inch to one-third inch square and of Parasitology iii 2
    No text description is available for this image
    No text description is available for this image
    No text description is available for this image