Note upon the presence of amido acids in the blood and lymph as determined by the Beta naphthalinsulphochloride reaction / by W.H. Howell.

  • William Henry Howell
Date:
[1906?]
Catalogue details

Licence: Public Domain Mark

Credit: Note upon the presence of amido acids in the blood and lymph as determined by the Beta naphthalinsulphochloride reaction / by W.H. Howell. Source: Wellcome Collection.

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    tinued for a long period. In the present experiments the membrane was constructed in the form of a flask with a capacity of about 250 c.c. For this purpose one takes a 5 to 6 per cent solution of gun cotton in a mixture of equal parts of absolute alcohol and ether. Some of the solution is poured into an Erlenmeyer flask of the desired capacity, and is then turned out while the flask is kept in slow rotation, so as to form an even layer of the adherent solution over the entire wall. The flask is drained carefully to remove the superfluous collodium, and is rotated slowly in the hands for several minutes until the solution adher- ent to the walls has hardened superficially. The flask is then filled with water; the membrane of collodium is separated at the mouth of the flask by means of a knife, and is gently detached from the walls of the flask for a distance by means of a blunt glass rod or spatula. After emptying the flask the entire membrane may then be removed conveniently by allowing a stream of water to flow in between the membrane and the glass wall. The membrane thus loosened may be pulled out without danger of rupturing. It presents the form of the Erlenmeyer flask, and may be kept in distilled water until used. In using these flasks to dialyze blood a glass tube 30 to 40 cms. in length was cemented into the mouth with collodium solution and firmly tied. By this means one prevents the overflow of the flask which would otherwise occur in consequence of the permanent osmotic pressure exerted by the blood. The blood was allowed to flow directly from the blood vessel used into the flask, which was then swung in a vessel containing two to four litres of distilled water, and the dialysis was continued for five to twenty-four hours. The dialysate was then concentrated by evaporation to 50 to 100 c.c., and tested directly with the ethereal solution of @ naphthalinsulphochloride, made alkaline according to the usual procedure by the addition from time to time of appropriate amounts of a normal solution of potassium hydroxide. The mixture was kept in the shaking apparatus for four to twenty- four hours. A positive reaction for amido acids was obtained in all cases but one in blood taken from fourteen different animals. The experiments were all made upon dogs, and the blood was collected either from the portal vein or from the carotid artery or jugular vein in animals that had been well fed, five to six hours after the meal, or in animals in a fasting condition, twenty-four to fifty hours after a meal. Examination of the dialysate.— After a dialysis of five to six hours the dialysate was usually entirely clear ; after a longer dialysis, how- é
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