An introduction to recombinant DNA / Alan E.H. Emery.

Alan Emery

Date:: [1984], ©1984

Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: An introduction to recombinant DNA / Alan E.H. Emery. Source: Wellcome Collection.

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$212 Recombination Cross-over between two linked loci. Recombination fraction (0) Frequency of recombination. Regulator gene A gene which synthesizes a repressor substance which inhibits the action of a specific operator gene. Repetitive DNA DNA sequences of variable length which are repeated up to 100,000 (middle repetitive) or over 100,000 (highly repetitive) copies per genome. Repression Inhibition of gene transcription. Restriction endonucleases Group of enzymes which cleave DNA. Type II enzymes cleave DNA at sequence- specific sites. Restriction fragment DNA fragment produced by a restriction endonuclease. Restriction fragment length polymorphism (RFLP) Polymorphism due to the presence or absence of a particular restriction site. Restriction map Linear arrangement of various restriction enzyme sites. Restriction site Base sequence recognized by a restriction endonuclease. Retrovirus RNA virus which replicates via conversion into a DNA duplex. Reverse transcriptase An enzyme which catalyses the synthesis of DNA from RNA. RFLP See restriction fragment length polymorphism. Ribosomes Minute spherical structures in the cytoplasm. They are rich in RNA and the seat of protein synthesis. RNA (= ribonucleic acid) The nucleic acid which is found mainly in the nucleolus and ribosomes. Messenger RNA transfers genetic information from the nucleus to the ribosomes in the cytoplasm and also acts as a template for the synthesis of polypeptides. Transfer RNA transfers activated amino acids from the cytoplasm to messenger RNA. RNA polymerase ^ An enzyme which catalyses the synthesis of RNA in transcription. SI nuclease An enzyme which degrades single-stranded DNA.$

An introduction to recombinant DNA / Alan E.H. Emery.

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