Genetic control of natural resistance to infection and malignancy / edited by Emil Skamene, Patricia A.L. Kongshavn, Maurice Landy.

  • International Symposium on the Genetic Control of Natural Resistance to Infection and Malignancy (1980 : Montréal, Québec)
Date:
1980
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Genetic control of natural resistance to infection and malignancy / edited by Emil Skamene, Patricia A.L. Kongshavn, Maurice Landy. Source: Wellcome Collection.

    596/632 (page 570)
    570 Mary M. Stevenson et al Figure 2. Chemotaxis of peritoneal macrophages dose re¬ sponse to EAMS. Peritoneal exudate cells were collected from untreated mice or mice treated ip with thioglycollate 3 days previously. Chemotactic responses were assayed to endotoxin-activated mouse serum (EAMS) by standard methods (9). Response is expressed as mean number of macrophages per number of fields shown ± standard error of mean for triplicate filters. fact, most obvious at the highest dilution (1/900) of EAMS tested. In addition, the difference in chemotactic re¬ sponsiveness was not related to the time of incubation (Fig. 3). With macrophages from either strain, there was accelerated Chemotaxis between 0 hr and approximately 3-4 hrs and a plateau in the response by 6 hrs. In spite of the similarity in kinetics, macrophages from BIO.A mice treated with thioglycollate were more responsiveness to 1/100 dilut¬ ion of EAMS at each time point assayed. The cells responsible for the enhanced macrophage chemotactic responsiveness were evident within 2 days of i.p. injection of BIO.A mice with thioglycollate and remain¬ ed evident through 4 days (Fig. 4) . Macrophages from A/J mice did not show enhanced chemotactic responsiveness