Transplantation genetics of primates / editors Hans Balner, Jon J. van Rood.

Date:
[1972]
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Transplantation genetics of primates / editors Hans Balner, Jon J. van Rood. Source: Wellcome Collection.

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    72 DORF, EGURO, AND AMOS 2Ь H TESTED ON. HL-A 11 CELLS -:k. (2) ^0(1) -p(3) ^(3) aHL-AII ÛHL-A1 OHL-A10 OHL-АЗ • NEGATIVE TESTED ON HL-A 1 CELLS ■■•■f.... -4(2) TESTED ON HL-A 10 CELLS K] NUMBER OF ABSORPTIONS Fig. 1. Serial absorptions using 2 x 10® platelets per absorption. Numbers in paren¬ theses indicate the number of different plate¬ let donors with the stated HL-A specificities used for absorptions. The second HL-A antigen present on cells of these donors was not HL-A 1, 10, or 11. Vertical lines indicate the maximal range of residual antibody ac¬ tivity remaining following absorptions. Anti¬ body scores are determined by summing the reaction scores (+ to ++++) during titra¬ tions of absorbed sera with at least three donors with the stated HL-A specificity, the which donor Anderson's platelets were extracted was delayed 1 wk in transit, and arrived in unsatisfactory condition. Additional absorptions were performed with platelets from four chimpanzees, two gibbons, and an orangutan (Fig. 2). Plate¬ lets from chimpanzees Melilot and Sandra could completely absorb all cytotoxic ac¬ tivity from serum Anderson. In contrast, platelets from chimpanzee Edgar removed cytotoxins for HL-A 10 and HL-A 1 cells, but not for HL-A 11 lymphocytes. Absorp¬ tions with platelets from chimpanzee Homer readily cleared HL-A 10 cytotoxins, removed anti-HL-A 1 activity with some difficulty, but gave only slight absorption of HL-A 11 activity. Platelets from gibbon Ajay absorbed serum Anderson in the same manner as those from chimpanzee Homer. The absorption patterns with cells from a second gibbon, Blackey, resembled those of chimpanzee Edgar. Orangutan platelets were the least efficient for absorption of HL-A 11, HL-A 10, or HL-A 1 activity from this serum. results are then averaged. Table 1* Reaction of Senoi Anderson Vlth Hvoian I^phoc7tee Iridio ctye Donore Reclpxx)cal of hlghMt dilution glTlng a posltlTe reaftlon HL-A 1-11 variant antigen HoMozygouB HL-A 3 donor Ten additional donors, negative with all bleedings of serum Anderson and lacking IIL-A, 11, 10, 1 or 3 are not illustrated.