Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians / by T.H. Pearmain and C.G. Moor.

  • Moor, C. G. (Cresacre George)
Date:
1898
Catalogue details

Licence: Public Domain Mark

Credit: Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians / by T.H. Pearmain and C.G. Moor. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    anaerobic bacteria. The tubes for this purpose are filled two-thirds full. (11) Urine Gelatine and Agar.—Fresh urine thickened with 10 per cent, of gelatine, or 2 per cent, of agar, with the addition of 1 per cent, of peptone and ^ per cent, of sodium chloride, is rendered feebly alkaline and filtered. The details of the method of preparation are the same as those already described for nutrient agar and gelatine. These two media are largely used in Germany, and are said to yield equally satisfactory results to those prepared from broth. (12) Peptone Solution.—Ten grammes of peptone and five grammes of sodium chloride are dissolved in 1,000 c.c. of distilled water; the solution is then w'ell boiled, and neutra- lised carefully in the usual manner. The solution is again boiled and filtered. The solution is then run into tubes, and steamed for fifteen minutes on three successive days. These tubes are used in the diagnosis for cholera. (13) Milk-tubes.—‘ Separated ’ milk is carefully neutra- lised with sodium bicarbonate and filled into tubes, and sterilised as usual. These tubes are useful for the differ- entiation between typhoid and coli. (14) Potato-tubes.—Large and sound potatoes are tho- roughly scrubbed until clean, and then with a large cork- borer cylmdrical pieces are bored to fit into test-tubes. Each cylmder is cut into two halves diagonally, and each half placed in a test-tube. It is advisable to allow the cores of potato to rest on a moist plug of cotton-wool; this will keep the potato-cylinder moist. The tubes are then plugged and capped as usual, and sterilised for thirty minutes in the steam steriliser on three successive daj’s. Potatoes form an excellent culture medium for many organisms, and one which secures their development in a very characteristic way. Care should be taken to prevent