Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians / by T.H. Pearmain and C.G. Moor.

  • Moor, C. G. (Cresacre George)
Date:
1898
Catalogue details

Licence: Public Domain Mark

Credit: Applied bacteriology; an introductory handbook for the use of students, medical officers of health, analysts and sanitarians / by T.H. Pearmain and C.G. Moor. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    serum is rendered faintly alkaline and run off into test- tubes, which are then plugged as usual, and laid on a slanting surface, and the serum made to set by heating in the hot-air steriliser to 75° C. The tubes can then be steiilised in the usual way by steaming on three successive occasions. We have found the above to be the most easy method of preparing hlood serum, and one which gives peifectly satisfactory results. The serum, when so piepared, should have a jelly-like consistency, and is of an opalescent, yellowish-white colour. The serum from human blood, which is sometimes to be obtained at operations and from placentae, is used by some workers, but its use presents no advantages over that obtahied from the horse or other animals. Modifications of Blood Serum.—The fluid obtained from hydroceles, cysts, or dropsical effusions is practically the same in composition as blood serum, and the method of preparing nutrient media from it is the same as in the case of blood serum. (18) Lo’fller’s Medium.—This medium, which is used for rapid diagnosis of diphtheria, is prepared as follows : Two parts of blood serum are mixed with one part of nutrient grape-sugar broth. The tubes are then solidified in a slanting position, and treated as in the case of the ordinary serum tubes. (19) Egg Albumin.-—The albumin from birds’ eggs is a very convenient and good medium for the growth of many bacteria. The albumin is carefully separated from the yolk, and treated as directed under the preparation of blood serum tubes. The white from plovers’ eggs yields an almost transparent medium. Hens’ eggs may, according to Hiippe, be themselves used with advantage as nutrient media. The newly-laid eggs are washed in soda solution, and then laid in 1 in 2,000 mercury bichloride solution for a