Influence of excess vitamin A on the sulphate metabolism of chick ectoderm grown in vitro / by H.B. Fell, Sir Edward Mellanby, and S.R. Pelc.

  • Fell, Honor B.
Date:
[1954?]
Catalogue details

Licence: In copyright

Credit: Influence of excess vitamin A on the sulphate metabolism of chick ectoderm grown in vitro / by H.B. Fell, Sir Edward Mellanby, and S.R. Pelc. Source: Wellcome Collection.

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    Reprinted from the British Medical Journal September 11, 1954, vol. ii, p. 611 INFLUENCE OF EXCESS VITAMIN A ON THE SULPHATE METABOLISM OF CHICK ECTODERM GROWN IN VITRO BY H. B. FELL, D.Sc., F.R.S. Strangeways Research Laboratory, Cambridge Sir EDWARD MELLANBY, G.B.E., K.C.B., F.R.S. National Institute for Medical Research, London AND S. R. PELC, D.Phil. Medical Research Council, Experimental Radiopathology Unit, Hammersmith Hospital, London [With Special Plate] In previous experiments (Fell and Mellanby, 1953) it was shown that when the presumptive epidermis from 6-7-day chick embryos is grown in medium containing excess vitamin A ( + A medium) it differentiates, not into the squamous, keratinizing epithelium of the skin as in normal medium but into a mucus-secreting, often ciliated, membrane resembling that of the nose. Re¬ cently an attempt has been made to investigate some of the biochemical changes which accompany this meta¬ plasia, by studying the uptake of radioactive sulphur given in the form of the sulphate (Na235SQ4) in ecto¬ derm grown in normal and in + A medium. Singher and Marinelli (1945) found that when labelled sulphate was injected into rats it was incorporated into chondroitin sulphate and related compounds ; its in ¬ corporation into amino-acids was negligible in animal though considerable in plant tissues (Howard and Pelc, 1951). Dziewiatkowski et al. (1949) confirmed these findings in cartilage. Bostrom et al. (1952) demon¬ strated the uptake of sulphate in cartilage and also in mucous epithelium by means of autoradiographs, though the resolution was not sufficient to allow detailed in¬ vestigation of the distribution and movement of the 559/54