On pancreatic lipase. IV. The action of serum on mice inoculated with malignant mouse tumour / by J.A. Shaw-Mackenzie.

  • Shaw-Mackenzie, John A. (John Alexander), 1857-1933.
Date:
1911
Catalogue details

Licence: In copyright

Credit: On pancreatic lipase. IV. The action of serum on mice inoculated with malignant mouse tumour / by J.A. Shaw-Mackenzie. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    'y<p Journal^ Physiology, Vol. \From ths Ptocoodings of-ihs Physiologicol SoctBty, Jan. 21, 1911.] - - /J2.* On pancreatic lipase; IV. The action of serum of mice inoculated with malignant mouse tumphr. By J. A. Shavv- Mackenzie. In previous communications(i) made in conjunction with Dr O. Rosenheim we noted that serum, although not possessing any fat- splitting (lipoclastic) action by itself, nevertheless increases the activity of pancreatic lipase. In cases of carcinoma in the human subject and in certain patho- logical conditions, however, we made the observation that this reaction of the serum was increased in a remarkable way; whilst cholesterin exerted in all cases an inhibitory influence. The present observations refer to the action of serum (1) of normal mice; (2) of mice which have been inoculated with mouse tumour and presenting progressive growths; (3) of mice which have recovered spontaneously from large growths; (4) of mice which have proved negative to inoculation with mouse tumour; (5) of mice which it is assumed have been rendered immune to subsequent inoculation with mouse tumour by previous injection with normal mouse-tissue emul- sions ; and (6) of mice which have been injected subcutaneously with some animal tissue extracts and substances. Details of technique have been published already. Briefly, it may be explained that the lipoclastic action is estimated by the amount of decinormal potash used for the neutralisation of the fatty acids set free by pancreatic lipase acting on olive oil emulsion; the mixture being incubated at a temperature of 37° C. for a certain time, usually 18 hours. Taking now normal mouse serum, the following table gives the results in a typical case; 0 7 c.c. of glycerin extract of pancreas with water, in the proportion of 1-2 and 2 5 c.c, of olive oil emulsion were mixed, and after incubation the amount of acid liberated was estimated. TABLE I. Average tuyrmaX mouse serum. Pancreatic extract alone on olive oil emulsion C.c.^KOU 6-1 Acceleration Addition of serum to extract 0-1 c.c. 8-2 + 2-1 0-25 „ 9-2 -t 3-1 0-6 „ 10-1 + 4-0