Studies on the etiology of blackwater fever / I.J. Kligler.
- Israel Jacob Kligler
- Date:
- 1923
Licence: Public Domain Mark
Credit: Studies on the etiology of blackwater fever / I.J. Kligler. Source: Wellcome Collection.
5/10 page 207
Licence: Public Domain Mark
Credit: Studies on the etiology of blackwater fever / I.J. Kligler. Source: Wellcome Collection.
![marked hemolysis when acting in combination. These experi- ments were repeatedly made with human red cells both normal and malarial as well as with rabbit cells, always with the same results. The following are type experiments indicating the method of procedure, concentrations, and results. Experiment 1. Ox bile was diluted with saline in the proportion of 1:9 and varying quantities added to a series of tubes containing the same amount of quinine. The volume in each case was made up to 1 cc., and 0.1 cc. of defibrinated blood added to each tube. The tubes were placed in the incubator for one hour and then observed at room temperature. Quinine mgm......| 0.5 0.5 a OF to 0.8f 90.5 0.0 Bue (1:10), ces. 0.1 0.2 0.3] 0.4) 0.5] 0.6) 0.0 0.3 Ne pee PUCSULES FRE S. No he- | Hemolysis | Immediate No he- | No he- molysis} in#4hour hemolysis molysis| molysis Experiment 2. Same procedure as experiment 1, except bile con- centration constant and quinine varied. Quinine mgm....... 0.4 0.2 0.1 0.05 0 Pieris 25) Co... eS. 1.0 1.0 1.0 1.0 1 pS nd Se ab at ale Eo LEO ala aS Hemolysisin five | Hemolysis in | Complete | Slight he- minutes in ten min- hemoly-| moly- utes sis one sis one hour hour Experiment 3. Effect of bile and quinine on blood of malaria patient. Blood taken at end of chill; serum yellowish. One-tenth cubic centi- meter defibrinated blood added to each tube containing 1 ce. of bile- quinine mixture. Tubes incubated one hour at 37°C., then left at room temperature ten hours. BICC OO ICG. 200 NG. wahiey sol take at 10:0) B.OKh) 4b0. EO 0.0 0.0 CISA OTR Eig os oa etal dy ai eid a Oia Se 0.2; 0.3] 0.4} 0.0 2.0 0.0 Results, (per cent of cells laked)....]| 20 30 35 4.0| Trace} 0.0](https://iiif.wellcomecollection.org/image/b33439266_0005.jp2/full/800%2C/0/default.jpg)
